. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Nitro and Azo Dyes 97 like it thus recognized being C. 1. No. 353 (chlorazol fast pink 4BL) which differs from it primarily in that it is not methylated, has two hydroxyl groups attached to the naphthalene rings, and two sulfonic groups attached to the benzene rings. It has been called for in certain vital staining procedures, having been intro- duced for that purpose by Evans and Scott in 1921. u65 NIAGARA BLUE 4B C. I. NO. 510
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. Nitro and Azo Dyes 97 like it thus recognized being C. 1. No. 353 (chlorazol fast pink 4BL) which differs from it primarily in that it is not methylated, has two hydroxyl groups attached to the naphthalene rings, and two sulfonic groups attached to the benzene rings. It has been called for in certain vital staining procedures, having been intro- duced for that purpose by Evans and Scott in 1921. u65 NIAGARA BLUE 4B C. I. NO. 510 Synonyms: Pontamine sky blue 5BX. Direct sky blue. Benzo sky blue. OH OH NH, \ I N = N_/~\^/~\_N = N. ./ \/\ OCH3 OCH3 NaS03 S03Na NaSOi SOjNa C34H24N60i6S4Na4; Mol. Wt. {An acid dye; absorption maxima about 6SJ!t,.2 and ) Occasional references have been made to this dye in connection with histology for some time, although it has never come into general use. Recently Varrelman (1938) has employed it for demonstrating the vascular system of plants, taking advantage of its rise through stems which are stood in a solution of the dye. In the vital staining of skin or of tumors a pontamine sky blue has been recommended, which may be this dye or may be C. I. No. 518 which has been sold under the name of pontamine sky blue 6BX (Syn. Niagara sky blue 6B or direct sky blue). d68 MARSHALL RED d70 HICKSON purple These are two new dyes of unknown composition, but stated to belong to the disazo group. They are discussed by Cannon (1941) as having been manufactured in the course of his study of chlorazol black E and of dyes of other colors found to be associated with that dye. He claims that they are valuable nuclear stains in general histology. d75 chlorazol black E C. I. NO. 581 Synonyms: Pontamine black E, EX, or EXX. Erie black GXOO, B, BF. Direct black MS, RL, E, GX, EE, 2V, F or A. Direct deep black EW extra, E, EA, EAD extra. Renol black Please note that these ima
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