. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. mAb 1305-21 Ab 483-576 Blocked. 0) I I o ro O 5 201- 483-576 Blocked KigureS. Specificity of Na-channel antibodies determined with immunoblots of samples of giant fiber lobe (GFL), cleaned giant axon. cornea, and mantle muscle. (A) mAbm, was used as undiluted hybrid- oma supernatant. Secondary goat anti-mouse antibody dilution was 1:5000. (B) AbJKM76 was used at 1: 1000 dilution, while dilution of secondary goat anti-rabbit antibody was 1 (C) Ab48v!7t, used at I: 2000. dilution of secondary goat anti-rabbit antib
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. mAb 1305-21 Ab 483-576 Blocked. 0) I I o ro O 5 201- 483-576 Blocked KigureS. Specificity of Na-channel antibodies determined with immunoblots of samples of giant fiber lobe (GFL), cleaned giant axon. cornea, and mantle muscle. (A) mAbm, was used as undiluted hybrid- oma supernatant. Secondary goat anti-mouse antibody dilution was 1:5000. (B) AbJKM76 was used at 1: 1000 dilution, while dilution of secondary goat anti-rabbit antibody was 1 (C) Ab48v!7t, used at I: 2000. dilution of secondary goat anti-rabbit antibody was 1:5000. Samples were prepared as described and separated on 5't SDS-PAGE gels. Each lane was loaded with lO^g of total protein. Following transfer and antibody incubation, bands were detected by chemiluminescence reagent. A 260-280 kD band is detected in GFL and clean axon by monoclonal (A) and polyclonal (B) antibodies. A specific 210-220 kD band is present in mantle muscle, but not in cornea tissue (C). Blocking control was carried out as described in Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
