. Biochemical systematics. Biochemical variation; Botany. GENERAL EVALUATION 329. Fig. 16-1. Influence of crude extract from Baptisia leucophaea leaves upon Rf value of a pure sample of hydroxylupinine. The samples are co-chromatographed so as not to com- pletely overlap. Thus, the last block on the right has 8 appHcations of hydroxylupinine and no Baptisia extract; the next block has 7 applications of hydroxylupinine and one application of Baptisia extract. A single application of the Baptisia extract is sufficient to completely alter the Rf of the pure alkaloid. The lower band is a second al
. Biochemical systematics. Biochemical variation; Botany. GENERAL EVALUATION 329. Fig. 16-1. Influence of crude extract from Baptisia leucophaea leaves upon Rf value of a pure sample of hydroxylupinine. The samples are co-chromatographed so as not to com- pletely overlap. Thus, the last block on the right has 8 appHcations of hydroxylupinine and no Baptisia extract; the next block has 7 applications of hydroxylupinine and one application of Baptisia extract. A single application of the Baptisia extract is sufficient to completely alter the Rf of the pure alkaloid. The lower band is a second alkaloid present in the Baptisia extract. (Brehm, 1962). upon the method of preserving, extracting, and chromatographing the sample. It is likely that such artifacts are more important than is generally recognized. Extensive changes following the harvesting of plant material have been noted variously (Yoshida, 1961) but do not necessarily occur (Dzhemukhadze and Nestyuk, 1961). Forsyth (1952) found that 80 per cent of the total polyphenols in cacao beans were removed within fifteen minutes when the beans were ground and aerated in a buffered solution. Although conditions were probably nearly optimal for the activity of oxidative enzymes, it is nevertheless important to recognize that many chemical changes may occur follow- ing harvesting, particularly influenced by the conditions at the time of death of the tissues. Volatile constituents are especially susceptible. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Alston, Ralph E; Turner, B. L. (Billie Lee), 1925-. Englewood Cliffs, N. J. , Prentice-Hall
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