. The Biological bulletin. Biology; Zoology; Marine biology. GLYCOPROTEINS OF SEA URCHIN EGG SURFACE 427 The sedimentation velocity of FP was not analyzed because the chemical analysis of this substance revealed it to be a mixture. Chemical analysis The amino acid content was found to represent , and in dry weight of the jelly, TFP and FP, respectively (Table II). Jelly substance, TFP and FP differ greatly among each other in their total contents of amino acids, but they contain the same amino acids in approximately the same proportions. As- partic and glutamic acids are predomi
. The Biological bulletin. Biology; Zoology; Marine biology. GLYCOPROTEINS OF SEA URCHIN EGG SURFACE 427 The sedimentation velocity of FP was not analyzed because the chemical analysis of this substance revealed it to be a mixture. Chemical analysis The amino acid content was found to represent , and in dry weight of the jelly, TFP and FP, respectively (Table II). Jelly substance, TFP and FP differ greatly among each other in their total contents of amino acids, but they contain the same amino acids in approximately the same proportions. As- partic and glutamic acids are predominant in both TFP and FP, whereas there 7 -. I/S CONCENTRATION {%) Figure 1. Sedimentation coefficient of jelly coat substance. Solid circles: Svedberg unit (S) plotted against concentration of jelly. Open circles: Reciprocal of S values plotted against concentration of jelly. Conditions, see text. is relatively more isoleucine, leucine and phenylalanine in the jelly substance. There is less proline, glycine, alanine, cysteine, tyrosine and arginine in the jelly than in TFP and FP. The large amounts of cysteine or cystine suggest that the constituent proteins have a complex structure, especially in TFP whose cystine was not completely cleaved even under a degree of hydrolysis which destroyed hexosamine. The N-terminal amino acids of the three substances are listed in molar ratio in Table III. This table shows that the amino acid-containing con- stituents of TFP and FP are mixtures of polypeptides or proteins. The N-ter- minal amino acids of jelly substance consist of two moles of phenylalanine and one mole of arginine; no minor components could be detected. This result suggests that the jelly is composed of three polypeptide Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Lillie,
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