. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 N. J. BERRILL It has already been shown (Berrill, 1929) that the membranes are normally digested from within by a proteolytic enzyme secreted by the developing tadpole, that the enzyme is fairly stable and is active within the limits pH Trypsin digests the membranes, but only very slowly, so that the protease concerned is probably a tryptic ferment of a less specialized nature than trypsin itself. There are, accordingly, two means by which membranes of ascidian eggs may be removed. The enzyme produced by develo
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 382 N. J. BERRILL It has already been shown (Berrill, 1929) that the membranes are normally digested from within by a proteolytic enzyme secreted by the developing tadpole, that the enzyme is fairly stable and is active within the limits pH Trypsin digests the membranes, but only very slowly, so that the protease concerned is probably a tryptic ferment of a less specialized nature than trypsin itself. There are, accordingly, two means by which membranes of ascidian eggs may be removed. The enzyme produced by developing ascidian tadpoles may be collected and concentrated, or a suitable and more readily obtainable substitute may be found. It was discovered that dona, Phallusia, Ascidia, and Ascidiella eggs remained about 100 per cent viable at 18° C. for a little over twenty-four hours, so that any enzyme mixture to be of use must be effective within that Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
Size: 1962px × 1274px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
