Archive image from page 149 of The cytoplasm of the plant. The cytoplasm of the plant cell cytoplasmofplant00guil Year: 1941 Fig. 85. — Vital staining with neutral red, except 03, observed under the microscope. A, PeniciUium glaucum. 1, before staining; 2, small deeply stained precipitates in the vacuole showing Brownian movement; 3, fusion of small precipitates to larger bodies; 4, precipitates appressed to peripheral wall of vacuole, diffuse staining of sap. B, Zygosaccharomyces Chevalieri. 1, small precipitates in vacuole; 2, 3, fusion, bodies now appressed to wall of the vacuole, sap dif
Archive image from page 149 of The cytoplasm of the plant. The cytoplasm of the plant cell cytoplasmofplant00guil Year: 1941 Fig. 85. — Vital staining with neutral red, except 03, observed under the microscope. A, PeniciUium glaucum. 1, before staining; 2, small deeply stained precipitates in the vacuole showing Brownian movement; 3, fusion of small precipitates to larger bodies; 4, precipitates appressed to peripheral wall of vacuole, diffuse staining of sap. B, Zygosaccharomyces Chevalieri. 1, small precipitates in vacuole; 2, 3, fusion, bodies now appressed to wall of the vacuole, sap diffusely stained. C, Saccharomyces ellipsoideus; 1, 2, as in A, 1-2; 3, cells fixed by formol stained with cresyl blue which causes flocculation of the metachromatin from the colloidal substances in the vacuole as numerous, deeply stained bodies. the chondriosome and leucoplasts but it stains them faintly. It is almost immediately reduced to its rose derivative, however, and this diffuses into the cytoplasm, the nucleus, or the vacuole, and its concentration is too weak to produce in them any appreciable coloration. On the contrary, a solution of the dye taken up by the chondriosomes and leucoplasts, is reduced to its rose deriva- tive which, being less strongly retained by these elements, diffuses into the cytoplasm and nucleus which it colors a pale rose in cells whose vacuoles are lacking in oxyflavanol compounds. In the case of the vacuole which, on the contrary, contains oxyflavanol com- pounds and has accumulated Janus green, reduction goes on at the same time in the chondriosomes and in the vacuole, and a part of the rose derivative formed in the chondriosomes diffuses into the vacuole. Often indeed, the reduction begins in the vacuole and takes place later in the leucoplasts.
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