. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 MILTON FINGERMAN AND K. RANGA RAO decreased and completely disappeared in the samples heated for 12 hours and longer. However, concomitantly the white pigment-concentrating activity began to appear and gradually increased with decrease of the white pigment-dispersing activity. In contrast, the material in the extract prepared directly in water always evoked only dispersion of the white pigment. Even if the white pigment-concen- trating substance is present in the material extracted directly in water, it is not possible t
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 210 MILTON FINGERMAN AND K. RANGA RAO decreased and completely disappeared in the samples heated for 12 hours and longer. However, concomitantly the white pigment-concentrating activity began to appear and gradually increased with decrease of the white pigment-dispersing activity. In contrast, the material in the extract prepared directly in water always evoked only dispersion of the white pigment. Even if the white pigment-concen- trating substance is present in the material extracted directly in water, it is not possible to demonstrate its activity by this method because the antagonistic white pigment-dispersing substance in this fraction is thermostable. 25 - 20 10. 10 II 12 13 14 15 16 17 18 19 20 21 FRACTION NUMBER 2223 24 25 FIGURE 8. The white pigment-dispersing (dots and solid triangles) and white pigment- concentrating (circles and empty triangles) Standard Integrated Responses (SIR) evoked by the fractions of the ethanol-soluble material extracted from the eyestalks of Uca (dots and empty triangles) separated by filtration through a 28 X cm column of Sephadex LH-20. Flow rate : 30 ml per hour. Sample size: 2 ml. From the above experiment it is clear that the ethanol-soluble fraction of the eyestalks from Uca has both the white pigment-dispersing and -concentrating sub- stances. The ethanol-soluble fraction of the eyestalks from Palaeiuonetcs also has both substances (Fig. 2 ). However, the white pigment-concentrating substance did not appear in any of the fractions obtained after chromatographing the ethanol- soluble fractions of eyestalks from Uca and Palaemonetes on the Bio-Gel. Conse- quently in an effort to separate the white pigment-dispersing and -concentrating substances and obtain an estimate of their relative molecular weight it was de- cided to try another gel. Ethanol extracts were consequently chromatographed on Sephadex LH-20 with ethanol as the solvent. Fifty eyestalks
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
