. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HORMONES AND RETINAL PIGMENT 221 three groups of three pans containing 10 animals in each were given a one-minute, 250 flash of light at 6 and then returned to the darkroom. At the end of an hour in the dark when the animals were expected to be maximally light-adapted as a result of the flash, they were brought into 250 illumination again for ten minutes during which time one group was injected with extract of tritocerebral com- missure, the second group with an extract of eyestalk, and the third group wit
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. HORMONES AND RETINAL PIGMENT 221 three groups of three pans containing 10 animals in each were given a one-minute, 250 flash of light at 6 and then returned to the darkroom. At the end of an hour in the dark when the animals were expected to be maximally light-adapted as a result of the flash, they were brought into 250 illumination again for ten minutes during which time one group was injected with extract of tritocerebral com- missure, the second group with an extract of eyestalk, and the third group with sea water as a control. The extracts were prepared in the usual manner. Following injection the three groups of pans were returned to the darkroom and distal pigment indices of 10 animals in each group were determined at 30, 60, 150. 210 and 270 x LJ O h- Ld O C/) O O TRITO. COMM. O ES • SW 0 60 120 180 240 TIME IN MINUTES FIGURE 5. Comparison of the light- and re-dark-adaptational responses of prawns over- night in darkness to a simultaneous 250 light stimulus and injection of extracts or sea water. Trito. Comm.—tritocerebral commissure ; ES—eyestalk ; SW—sea water. minutes following the beginning of the second light period. Since there were only three containers of animals in each group, each container was returned to the dark- room after each distal pigment index determination so that it could be used for another determination. Thus, container 1 was used for the 30-minute and the 210-minute determination while container 2 was used for the 60-minute and the 270-minute determination. It had previously been determined that the brief ex- posure to the microscope light during a determination for animals in this experi- mental state was not significantly effective in inducing light-adaptation. This is substantiated also by the response of the control group of this experiment. Averages of the distal pigment indices of the 10 animals for each time that a de
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
