. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. APLYSIA INK 329 CHROMATOGRAPHY. 3 PIGMENTS BLUE PHYCOCYANIN PHYCOERYTHRIN PHYCOERYTHROBIUN (a.^.J-subunits)' Figure 5. Schematic representation of derivation of pigments of Aplyxia ink from phycobilisomes of a hypothetical red alga. Although not indicated on the diagram, B-phycoerythnn has phycourobilm only on the 7 subunit, while R-phycoerythrin has phycourobilin on both the 7 and (I subunits. C-Phycocyanin has only phycocyanobilms, while R-phycocyanin has both phycocyanobilin and phycoerylhrobilin. (For references, see Mac
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. APLYSIA INK 329 CHROMATOGRAPHY. 3 PIGMENTS BLUE PHYCOCYANIN PHYCOERYTHRIN PHYCOERYTHROBIUN (a.^.J-subunits)' Figure 5. Schematic representation of derivation of pigments of Aplyxia ink from phycobilisomes of a hypothetical red alga. Although not indicated on the diagram, B-phycoerythnn has phycourobilm only on the 7 subunit, while R-phycoerythrin has phycourobilin on both the 7 and (I subunits. C-Phycocyanin has only phycocyanobilms, while R-phycocyanin has both phycocyanobilin and phycoerylhrobilin. (For references, see MacColl and Guard-Friar, 1987.) Bromophenol Blue. The final concentration of ink was 100 Mg in the 20 n\ of heated sample which was applied to each lane. The gels were stained with Coomassie blue, and destained with 50% distilled water; 40% methanol; 10%' acetic acid. The gels were then stored in a 5% glycerol solution. Standards used for molecular weight calibration were: bovine serum albumin, ovalbumin, chymotrypsin- ogen, and lysozyme. Results and Discussion Chromophore content Visible absorption spectra ofAplysia inks (Fig. 1) show a number of bands. The relative intensities of the various band maxima vary with ink source; two typical results are shown (Fig. 1). Earlier studies on the chromophore content of the ink have yielded variable results (Christo- manos, 1955; Winkler, 1959; Chapman et al., 1967; Led- erer and Huttrer, 1942; Schreiber, 1929; Nishibori, 1960). We have, therefore, chosen to separate the ink chromo- phores by two entirely distinct methods: thin-layer (non- aqueous) and gel filtration (aqueous, pH ) chromatog- raphy. Thin-layer chromatography (TLC) separated the ink into three colored components. The fastest-migrating (Rf = ) was purple; the middle component (Rr = ) was blue; and the third, which remained near the origin (Rf = ), was red. When a TLC plate was run in two dimensions, a yellow component was also observed in the vicinity of the b
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
