Journal of bacteriology . 15 ACia 1 (culture R) Fig. 1. The relative size of colonies of B. lactis-acidi grown on plain and lac-tose agars. Plates were incubated at 37°C. for forty-eight hours. Graphs areplotted from the average of ten colonies from each medium. 485 486 JAMES M. SHEKMAN Lactose agar was used in all of the tests herein reported, butfrom the results of a number of comparisons it may be said thatglucose is apparently just as efficient as is lactose, both as tonumber and size of colonies. According to the work of Heine-mann and Glenn glucose and lactose agars are of equal value. (
Journal of bacteriology . 15 ACia 1 (culture R) Fig. 1. The relative size of colonies of B. lactis-acidi grown on plain and lac-tose agars. Plates were incubated at 37°C. for forty-eight hours. Graphs areplotted from the average of ten colonies from each medium. 485 486 JAMES M. SHEKMAN Lactose agar was used in all of the tests herein reported, butfrom the results of a number of comparisons it may be said thatglucose is apparently just as efficient as is lactose, both as tonumber and size of colonies. According to the work of Heine-mann and Glenn glucose and lactose agars are of equal value. (CultureBi) Fig. 2. Same as figure 1 except a different strain of B. lactis-acidi was used. for the quantitative bacterial analysis of milk, and since glu-cose is cheaper it should, perhaps, be recommended as the stand-ard for routine work. A limited number of trials have also * Jour. Infect. Dis., 5: 412-420. ADVANTAGES OF A CARBOHYDEATE MEDIUM 487 indicated that the amount of carbohydrate may be reduced per cent without impairing the value of the medium. Another advantage of a medium containing a carbohydrateover plain agar is that the former is of considerable value indifferentiating the types of organisms on the plates. The colo-nies of acid-producing bacteria on sugar agar are surrounded by
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