. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 400 C. LEMA-FOLEY ET N-cells + H o R-cells + H N-cells + Hp-r =¥=* 10 15 20 time (min) 25 30 Figure 8. Assay for hemolymph activity (H,) following cell-shape transformation and recovery, and test of capacity of recovered cells (CR) to undergo a second transformation. Upon recovery, R-cells reincubated with fresh hemolymph again underwent shape transformation, whereas reincu- bation of fresh N-cells in the "post-reversal" cell-free hemolymph (H^r) showed that it lacked H,. (Fig. 8, open circles). However, the c
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 400 C. LEMA-FOLEY ET N-cells + H o R-cells + H N-cells + Hp-r =¥=* 10 15 20 time (min) 25 30 Figure 8. Assay for hemolymph activity (H,) following cell-shape transformation and recovery, and test of capacity of recovered cells (CR) to undergo a second transformation. Upon recovery, R-cells reincubated with fresh hemolymph again underwent shape transformation, whereas reincu- bation of fresh N-cells in the "post-reversal" cell-free hemolymph (H^r) showed that it lacked H,. (Fig. 8, open circles). However, the converse was not true: N-cells incubated in hemolymph in which other cells had previously undergone shape transformation and recovery either remained normal or exhibited only partial transfor- mation and very rapid recovery, indicating depletion of Hx (Fig. 8. squares). Cytoskeletal structure and function As revealed by indirect anti-tubulin immunofiuorescence, the MB was still present and continuous in the cytoskeletons of shape-transformed cells, but its shape was highly convo- luted compared to that of normal cells (Fig. 9). To test whether the MB was a primary effector of shape transfor- mation, erythrocytes with and without MBs were prepared by temperature cycling in the presence of inhibitors of disassembly (taxol) or reassembly (nocodazole). Similarly cycled controls (DMSO solvent only) contained completely or partially reassembled MBs. Bioassays in diluted hemo- lymph produced more than 96% X-cells in all three prepa- rations within 10 min, and reversal after 3 h. One major difference was noted in cells lacking MBs, however: many of the major transformation-induced surface indentations were not eliminated during reversal (Fig. 10). Shape transformation inhibitors Heating hemolymph in a boiling water bath, followed by cooling and bioassay. showed Hx to be heat-labile. In three experiments in which controls showed very high initial. Please note that these images are extra
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
