. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 E. CARPIZO-ITUARTE AND M G. HADFIELD 30 mA/ TEA was tested in combination with a 3-h pulse of Cs* (Fig. 6B). Metamoiphosis was reduced, but to a lesser extent, when larvae were exposed simultaneously to a biofilm and to 10 or 15 mM TEA (P < ) (Fig. A. K +TEA Biol'ilm TEA Filtered seawalcr. ^ r^ Cs+ TCA Cesium Biofibu TEA Filtered scavvater r^n .^r^ C. =â 60 o E « L-S+.10 Cs»10 Cs»l Cs BIO 1(1 I" Treatment ITâI Biofilm+TEA Biot'ilni TEA I Flllered scau aler m ^ n n BI015 BK>*10 Bill 15 10 FSW Treatment Fi


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 20 E. CARPIZO-ITUARTE AND M G. HADFIELD 30 mA/ TEA was tested in combination with a 3-h pulse of Cs* (Fig. 6B). Metamoiphosis was reduced, but to a lesser extent, when larvae were exposed simultaneously to a biofilm and to 10 or 15 mM TEA (P < ) (Fig. A. K +TEA Biol'ilm TEA Filtered seawalcr. ^ r^ Cs+ TCA Cesium Biofibu TEA Filtered scavvater r^n .^r^ C. =â 60 o E « L-S+.10 Cs»10 Cs»l Cs BIO 1(1 I" Treatment ITâI Biofilm+TEA Biot'ilni TEA I Flllered scau aler m ^ n n BI015 BK>*10 Bill 15 10 FSW Treatment Figure 6. Inhibition of metamorphosis of Hydroides elegans by the ion channel blocker tetraethylammonium chloride (TEA). (A) Effect of TEA on metamorphosis in the presence or absence of 50 mM excess K* in seawater for 24 h. (B) Effect of TEA on metamorphosis in the presence or of a 3-h pulse of 10 mA-/ CsCI in seawater. (C) Effect of TEA in the presence or absence of a marine bioHIm for 12 h. Bars indicate mean percentages of larvae that metamorphosed ± 1 SE (;; = 4 replicates/treatment), evaluated 48 h after initial exposure to excess K*, 24 h after initial exposure to CsCI, and 12 h after initial exposure to a marine biofilm. K = 50 mM excess K* for 24 h: Cs = 3-h pulse of lOniM CsCI; 1, 10. 15 and 30 = mM concentrations of TEA; BIO = substratum coated with a marine biofilm; FSW = seawater filtered through filter. 100 75 50 -I 25 3 days old JL I 1 24 h [mam 48 h GO o O a c3 .*â» 100 75 50 25 0 100 75 50 25 0 100 75 50 25 4 days old i,g^ n ni 5 days old n T 6 days old ru 30 50 CsCI BIO FSW Excess K' (mM) Treatment Figure 7. Metamorphosis of 3- to 6-day-old larvae of Hydroides elegans from a single batch when exposed to different inducers. Larvae were removed from the 10 mM CsCI after 3 h and from 30 and 50 mA/ excess K* after 24 h. Bars indicate mean percentages of larvae that metamorphosed ± 1 SE (;i = 4 ). BIO =


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