. Laboratory work in bacteriology. Bacteriology. TUBING OF MEDIA. 511 drawn up into a sterile bulb pipette (Pig. 61), and then transferred direct to these tubes. The addition of sterile litmus solution to sterile media is preferable to the ordinary method of adding the litmus to the medium before steril- ization. Tubing of Media. In many experiments, as in the disinfection tests pres ently to be described, it is desirable to employ measured .amounts of bouillon. This can be done expeditiously by means of the simple apparatus shown in Pig. 72. The lower end of a 100 burette is attached to


. Laboratory work in bacteriology. Bacteriology. TUBING OF MEDIA. 511 drawn up into a sterile bulb pipette (Pig. 61), and then transferred direct to these tubes. The addition of sterile litmus solution to sterile media is preferable to the ordinary method of adding the litmus to the medium before steril- ization. Tubing of Media. In many experiments, as in the disinfection tests pres ently to be described, it is desirable to employ measured .amounts of bouillon. This can be done expeditiously by means of the simple apparatus shown in Pig. 72. The lower end of a 100 burette is attached to a small glass T-tube which connects with the reservoir flask. The burette can thus be readily filled and exact amounts can be measured out into the tubes. For rapid tubing of gelatin, bouillon, agar, etc., where it is not essential to employ a definite volume it is well to employ a funnel or glass globe which is connected by means of rubber tubing with a drawn-out glass tube. A Mohr's clamp is attached to the rubber tubing or in its stead a glass stop-cock may be used. The perforation in the latter should have a diameter of about 3 mm. Such an ap- paratus is obtained by disconnecting the . f}'^- ?? long rubber tube at a in Pig. 72. simple apparatus can, therefore, be em- ^^' ^' ^'^ ployed for ordinary tubing of media, or for measuring out definite Apparatus for tubing definite quantities Thi'5 °' media. For ordinary *° tubing disconnect at a. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr


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