. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 124 G. W. KIDDER AND V. C. DEWEY TABLE I Medium Generation time in hours Population per ml. at end of log. phase Maximum yield cells/ml. Population per ml. at 1 1 days Heated casein per cent + A 18,000 160,000 61,000 Heated casein per cent + A + B! 17,500 ' 182,000 82,000 Filtered heated casein 1,600 5,500 2,000 Filtered heated casein + B, 1,800 8,000 3,200 Filtered heated casein +A 42,000 100,000 57,000 Filtered heated casein + A + BI 38,000 110,000 86,000 A = heat and alkali treated al
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 124 G. W. KIDDER AND V. C. DEWEY TABLE I Medium Generation time in hours Population per ml. at end of log. phase Maximum yield cells/ml. Population per ml. at 1 1 days Heated casein per cent + A 18,000 160,000 61,000 Heated casein per cent + A + B! 17,500 ' 182,000 82,000 Filtered heated casein 1,600 5,500 2,000 Filtered heated casein + B, 1,800 8,000 3,200 Filtered heated casein +A 42,000 100,000 57,000 Filtered heated casein + A + BI 38,000 110,000 86,000 A = heat and alkali treated alfalfa extract; B, = thiamine 1 microgram/ml. All media contains salts and riboflavin ( micrograms/ml.). 2 4 tr uj 0. t/l UJ. // // »/ w ? 1 C e s> i i HEATED CASEIN FILTRATE, ii ii ii it ii ii ii ii ii 1 1 i + Bi + A + A + Bi 1 l 1 1 1 0 24 48 72 96 120 144 HO U RS 168 192 216 240 264 FIGURE 1 When dethiaminized alfalfa extract is added to the heated casein or to the filtered heated casein the response is striking. Rapid growth now occurs in the heated casein while in the filtered heated casein the generation time is reduced by nearly one-half and the population at the end of the logarithmic growth phase is in- 11 < ased from around 1,600 to over per nil. The maximum yield is increased from ahout 5,000 to approximately 100,000 per nil. and a much higher population is maintained for at least 11 days (over 50,000 as compared to 2,000 per ml.) (Table. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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