. The Eusporangiatae; the comparative morphology of the Ophioglossaceae and Marattiaceae. Ophioglossaceae; Marattiaceae. THE GAMETOPHYTE contents appear opaque and obscures more or less the centrally placed nucleus. A marked exception to the ordinary type of spore was that found in an undetermined species of Ophioglossum collected at Buitenzorg in Java. This was supposed to be O. moluccanum, with which it was growing, but a comparison with typical specimens of the latter species showed marked differences, the most striking being the spores, which were larger than in the type, had much less den
. The Eusporangiatae; the comparative morphology of the Ophioglossaceae and Marattiaceae. Ophioglossaceae; Marattiaceae. THE GAMETOPHYTE contents appear opaque and obscures more or less the centrally placed nucleus. A marked exception to the ordinary type of spore was that found in an undetermined species of Ophioglossum collected at Buitenzorg in Java. This was supposed to be O. moluccanum, with which it was growing, but a comparison with typical specimens of the latter species showed marked differences, the most striking being the spores, which were larger than in the type, had much less dense contents, and were espe- cially notable in that they had regularly two nuclei, a condition unique, so far as I know, among the ferns. (See Campbell 8, fig. 157.) The granular contents of the spores include numerous albuminous granules, together with more or less starch and oil. GERMINATION IN OPHIOGLOSSUM. The first successful attempts to germinate the spores of Ophioglossum were made by me in 1892, when ripe spores of the epiphytic 0. pendulum were collected in Hawaii and brought to California. This species was found in Hawaii, growing usually upon the trunks of tree ferns, and the spores were sown upon bits of the bark-like masses of roots, which in the commoner tree ferns of Hawaii (species of Cibotium) cover the trunk with a thick, felted mass. These masses of roots were kept in jars, and the spores were sown upon them, a good many of them germinating in course of time. The germination was very slow, the spores often remaining unchanged for months, and none of these young prothallia developed beyond the stage with three cells. This failure to develop further was undoubtedly due to the fact that they did not become infected with the mycorrhiza, which is essential to the full development of the prothallium. In 1906 ripe spores of the same species were collected in Ceylon and Java. In Ceylon spores were secured at the botanical garden in Peradeniya and in the Barrawa Reserv
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