. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 124 Biological Stains gressed fast, both because of the small number of biologists who employ it and because of the expense to manufacture; but it is hoped that eventually it will be as well standardized as the more commonly used stains. k40 new blue R C. I. NO. 909 Synonyms: Naphthol blue R. Fast blue 3R. Phenylene blue. Meldola's blue. Indin blue 2RD, I I CH3 \/\_N_/\ / 1 I I UN-CH3 \/~o-\/ \ CI C18H15N2OCI; Mol. Wt. {A
. Biological stains; a handbook on the nature and uses of the dyes employed in the biological laboratory. Stains and staining (Microscopy); Stains and Staining; Dyes. 124 Biological Stains gressed fast, both because of the small number of biologists who employ it and because of the expense to manufacture; but it is hoped that eventually it will be as well standardized as the more commonly used stains. k40 new blue R C. I. NO. 909 Synonyms: Naphthol blue R. Fast blue 3R. Phenylene blue. Meldola's blue. Indin blue 2RD, I I CH3 \/\_N_/\ / 1 I I UN-CH3 \/~o-\/ \ CI C18H15N2OCI; Mol. Wt. {A basic dye; absorption maxima about \622\, 573y \53S]) Occasional reference has been made to this dye in the biological literature, under the name of naphthol blue R, most recently by Lewis (1938) in staining fat drops in bacteria. k45 RESAZURIN O H0~\/~"0"^/=0 C12H7NO4; Mol. Wt. This is not a dye, but a colored compound that has interesting properties as an oxidation-reduction indicator. It is blue in its completely oxidized state, and upon reduction becomes pink and then colorless according to the following reaction: H -\/""0^/=0 YiO~\/-~0~\/=0 HO"~\/~0-X/~OH Resazurin Resorufin Hydroresorufin (blue) (pink) (colorless) Various shades between blue and light pink can occur. A spectral curve of resazurin is included in Fig. 13, p. 118 (graph 3). It was first suggested by Ramsdell et al. (1935) that it might be more valuable than methylene blue as an indicator of reduction in milk, partly because of its greater speed in showing reduction and. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Conn, H. J. (Harold Joel), 1886-1975. Geneva, N. Y. , Biotech Publications
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