. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). â j/\E' \zoPropano/. oio~ZO 30 W 50 eO 70 80 90 WO 0 10 80 30 W 50 BO 70 80 SO 100 IVafer â» % Figure 7. Effect on the R/s of IA A, IBA, IAN, andlAE of various percentages of acetonitrile {left) and isopropanol (right) in water. the possibility that ammonia might affect natural auxins more labile than lAE led us to start a new search for a good solvent to separate the acid auxins. Knowing that ammonia did not
. The chemistry and mode of action of plant growth substances; proceedings of a symposium held at Wye College, University of London, July 1955. Plant regulators; Auxin; Growth (Plants). â j/\E' \zoPropano/. oio~ZO 30 W 50 eO 70 80 90 WO 0 10 80 30 W 50 BO 70 80 SO 100 IVafer â» % Figure 7. Effect on the R/s of IA A, IBA, IAN, andlAE of various percentages of acetonitrile {left) and isopropanol (right) in water. the possibility that ammonia might affect natural auxins more labile than lAE led us to start a new search for a good solvent to separate the acid auxins. Knowing that ammonia did not change the R/s appreciably, we simply omitted it and used Z5opropanol+water (80;20) (Nitsch and Nitsch, Table 3 Hydrolysis of ethylindole acetate by ammonia on paper chromatograms All the chromatograms were equilibrated overnight, then run for about 8 hours at room temperature in the dark. Only ethylindole acetate (4 y) was applied to each chromatogram initially. The spots were revealed with either the Gordon and Weber or the Ehrlich reagent. Intensity of colour Solvent mixture viv) {Proportions in per cent lAE spot lAA spot Hexane H2O 28°o ammonia 80 20 _ + + + + 80 18 2 + + + + 80 16 4 + + + + + 80 10 10 + + + + + 80 4 16 â + + + + 80 â- 20 _!_ ^ + + woPropanol H,0 28% ammonia 80 20 _ + + + + â 80 10 10 + + + + â 80 5 15 + + + + â 80 â 20 + + 12. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Wye College; Wain, R. L; Wightman, F. (Frank). London, Butterworths Scientific Publications
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Keywords: ., bookcentury1900, bookcollectionbiodiver, booksubjectgrowthplants
