. Elementary biophysics: selected topics. Biophysics. Dilute starting culture of bacteria Growth period of several hours Concentrated final culture. Concentrated final culture distributed 1 ml per tube into many tubes Fig. 5. Bacteria are grown for a given period of time from a dilute starting culture to a concentrated final culture. This final culture is distributed in 1 ml aliquots into many tubes. These tubes are then sampled to determine the num- ber of mutant bacteria in each tube. unity to warrant the deduction that there are different mechanisms in- volved in producing the fluctuations
. Elementary biophysics: selected topics. Biophysics. Dilute starting culture of bacteria Growth period of several hours Concentrated final culture. Concentrated final culture distributed 1 ml per tube into many tubes Fig. 5. Bacteria are grown for a given period of time from a dilute starting culture to a concentrated final culture. This final culture is distributed in 1 ml aliquots into many tubes. These tubes are then sampled to determine the num- ber of mutant bacteria in each tube. unity to warrant the deduction that there are different mechanisms in- volved in producing the fluctuations which result in the deviations. As with the deviation ratio test, the actual conclusion is that by chance alone a ratio this large or larger will occur in a certain fraction of cases with the given number of independent measurements in each of the vari- ances. And, once again, the question of significance is decided on the basis of one chance in 20 being significant and one chance in 100 being highly significant. As an example, we take an investigation which was historically a turning point in the understanding of the mechanism of mutations. The appearance of a mutant cell is detected by placing the cells in an environ- ment in which the mutant property can be expressed. An illustration from bacterial genetics would be the appearance of resistance to a given anti-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Epstein, Herman T. (Herman Theodore), 1920-. Reading, Mass. , Addison-Wesley Pub. Co
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