Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . ate envelope. The centre, to which all the constituent threads ofa group converge, is occupied only by a very dense network of thisformation (fig. 148). The pear-shaped bodies above alluded to may be more clearly definedby staining with Weigerf s 30 process. For this purpose a solution ismade by adding together 20 cc. absolute alcohol, 5 cc. concentratedacetic acid, and 40 cc. of distilled water, and to the mixture so much ofthe so-called Trench extract of litmus as will give the fluid a dark-redcolour,
Clinical diagnosis : the bacteriological, chemical, and microscopical evidence of disease . ate envelope. The centre, to which all the constituent threads ofa group converge, is occupied only by a very dense network of thisformation (fig. 148). The pear-shaped bodies above alluded to may be more clearly definedby staining with Weigerf s 30 process. For this purpose a solution ismade by adding together 20 cc. absolute alcohol, 5 cc. concentratedacetic acid, and 40 cc. of distilled water, and to the mixture so much ofthe so-called Trench extract of litmus as will give the fluid a dark-redcolour, remaining ruby-red after repeated filtering (Wedls31 litmussolution). In this solution the cover-glass preparations are allowed toremain for an hour or so, then lightly rinsed with alcohol, and placedfor two or three minutes in a 2 per cent, gentian-violet fluid, whichshould be boiled before use, and filtered after cooling. If the specimen be now examined, it will be found that whilst thecentral mass of Actinomyces is colourless, the fungus threads are X>^ h <(? )K M. Fig. 148.—Preparation from same ease as last, stained by Grams method (eye-piece IV.,[objective, oil immersion ^,, Zeiss: and open condenser). stained a ruby-red. Baranski32 advocates staining with most cases, however, the character of this fungus can be determinedby a simple microscopical examination. The physical qualities of thepus, and the discovery in it of groups of Actinomyces or the club-shaped degeneration forms of the parasite, are points which will aid thediagnosis. In particular cases it may be necessary to resort to Gramsstaining method, and to observe the minute structure of the ultimatethreads of the fungus, as noticed above (comp. p. 409). Bujwidzz hasobtained pure cultivations of Actinomyces by means of Buchners M method(p. 448). Seen with the naked eye, these bear a close resemblance tocultivations of tubercle-bacillus. 5. Bacillus of Glanders.—The specifi
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