Collected papers . aining varying amounts of thepowder with equal amounts of substrate were allowed to digest forvarying lengths of time. In the first chart the abscissa represents timein days and the vertical ordinates thereto Jictivity measured as cubiccentimetres of deci-normal sulphuric acid. There are four curves of whichthe lowest represents the results obtained by allowing OOo gram of thepowder to act on equal amounts of substrate for varying times. Fourbottles containing identical amounts of substrate and powder were putinto the hot room at the same time. One of these was taken out ont
Collected papers . aining varying amounts of thepowder with equal amounts of substrate were allowed to digest forvarying lengths of time. In the first chart the abscissa represents timein days and the vertical ordinates thereto Jictivity measured as cubiccentimetres of deci-normal sulphuric acid. There are four curves of whichthe lowest represents the results obtained by allowing OOo gram of thepowder to act on equal amounts of substrate for varying times. Fourbottles containing identical amounts of substrate and powder were putinto the hot room at the same time. One of these was taken out ontlie expiration of the times mentioned in the chart and the nitrogen asammonia in the tannic acid filtrate estimated. The other three curvessimilarly represent activity at varying times of OT gram, 02 gram and i\ H. P>. r>KAI)LKY •21 n ()*4i grain respectively. They were oblainetl in the same way (exceptthat the curve lor 0 2 was wot continued so loiii: and theestimations were made at dilferunt 20 30 40 50 60 70 80 90 lOU 0 120 Chart 1. 1 1 1 1 1 1 ?^,^^^ ^!^^^ Chart 2. The second chart is compiled from the same figures and shows certainpoints more clearly. In it the abscissa represents the amounts of enzymewhile the vertical ordinatcs represent activity as cubic centimetres ofdeci-normal acid representing ammonia in filtrate. There are fourcurves at 15, 39, 87, 111 hours respectively showing etiect of increasingamounts of enzyme. The figures following the charts are the protocols of experimentsfrom which the charts are compiled. 214 Leucoprotease and Anti-leucoprotease Experiment 5. Powder No. 6. Time 2^ dayi 3. Substrate , casein 2-50/0 sol. in sodium carbont solution 0 25 *7^.Powder Casein sol. Time Nitrogen as of deci-normal sulph. acid in 50 of filtrate 005 gm. + 50 15 hrs. = -4-4 005 + 50 39 = 6-4 0-05 + 50 87 = 8-8 0-05 + 50 111 = 9-4 0 05 (heated) + 50 41 = 30 0-1 gm. + 50 15 hrs. = 5-9 01 + 50 39 = 9-8 01 + 50 87 = 12-5 01 + 50
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Keywords: ., bookcentury1900, bookdecade1900, bookpublisherlondon, bookyear190
