. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 216 CHARLOTTE P. MANGUM AND CHARLES D. COX with intensity of stimulation. Therefore, we have treated the response as if it were all-or-none, which is very nearly correct. With the exception of the temperature dependence experiments, recalcitrance was rarely encountered within the period described helow and designation of an acceptable criterion was unnecessary. In no case did the worms show less than 90% uniformity to qualitative tests, and 80% to quantitative tests. D o **~r \ ALANINE \ M ARENARIA EXTRACT. EGG ALBUMEN EGG A
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 216 CHARLOTTE P. MANGUM AND CHARLES D. COX with intensity of stimulation. Therefore, we have treated the response as if it were all-or-none, which is very nearly correct. With the exception of the temperature dependence experiments, recalcitrance was rarely encountered within the period described helow and designation of an acceptable criterion was unnecessary. In no case did the worms show less than 90% uniformity to qualitative tests, and 80% to quantitative tests. D o **~r \ ALANINE \ M ARENARIA EXTRACT. EGG ALBUMEN EGG ALBUMEN TIME (min) FIGURE 1. Currents generated by Diopatra cuprca. Feeding current given in response to clam extract and 10~a M amino acid solutions. Rejection response given to egg albumen. Small deflections reflect ventilation movements. Each increment of ordinate is 1 nil; each increment of abscissa is 1 min. All qualitative tests were made in running seawater, and ample time (ranging from 15 minutes to one hour, depending on the volume of the container) between tests was allowed to insure thorough renewal of the medium as well as subsidence of the response. It soon became apparent that the nutritional condition of the animal has a great influence on the response. In their natural habitat, worms have full guts during most months of the year (Mangum et al., 1968). Animals brought into the laboratory may not respond, or they may respond inconsistently for a short period. Our observations have been made only on worms which have been acclimated to laboratory conditions for a minimum of three clavs. Conversely,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray,
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