. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 334 HOPE RITTER, volts FIGURE 1. Polarograms of reduced glutathione: (A) X 10~3 M GSH in M KC1, (B) X 10~4 M GSH in a complex salt medium also containing yeast, blood serum and methyl- cellulose (sensitivity X 5; pH ). were L-cysteine, adrenochrome semicarbazone, ascorbic acid, sodium anthraquinone sulfonate, acetylcholine chloride, riboflavin, and sodium thioglycollate. A X 10~3 M solution of reduced glutathione (GSH) in a supporting electro- lyte of M KC1 at pH produced a polarogram (Cu
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 334 HOPE RITTER, volts FIGURE 1. Polarograms of reduced glutathione: (A) X 10~3 M GSH in M KC1, (B) X 10~4 M GSH in a complex salt medium also containing yeast, blood serum and methyl- cellulose (sensitivity X 5; pH ). were L-cysteine, adrenochrome semicarbazone, ascorbic acid, sodium anthraquinone sulfonate, acetylcholine chloride, riboflavin, and sodium thioglycollate. A X 10~3 M solution of reduced glutathione (GSH) in a supporting electro- lyte of M KC1 at pH produced a polarogram (Curve A, Fig. 1) closely resembling that of Curve A, Figure 2. Addition of gm. GSH to a gut content sample increased the wave height without significant change in the previously re- corded half-wave potential. The comparative method of wave height-concentration plots indicated X 10~4 M GSH in an isotonic medium of mixed salts, nutrients, and methyl cellulose results in a polarogram (Curve B, Fig. 1) almost identical to that recorded from the natural gut content (Curve A, Fig. 2). The Ilkovic equation, mentioned earlier, was not employed in the calculation of GSH concentration. Application of this equation would require a viscosity reading for hind-gut content, and this has not yet been determined. B. Chemical GSH produces a violet color in the presence of sodium nitroprusside. A posi- tive reaction occurs when a thin layer of dark brown Cryptocercus gut content is tested on a white porcelain surface. Although ascorbic acid does not react with nitroprusside, cysteine and possibly other sulfhydryl compounds effect the positive reaction (Patterson and Lazarow, 1953).. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Ho
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
