. Laboratory work in bacteriology. Bacteriology. POST-MORTEM EXAMINATION OP INFECTED ANIMALS. 275 the common putrefactive bacteria from the intestinal canal. Consequently, in delayed examinations, impure cultures are apt to be met with. Moreover, an uncertainty will exist as to whether the secondary invasion occurred during life, or after death. Hence, in case the animal cannot be exam- ined at once, it should be placed in a suitable vessel and kept on Fig. 48. a—Searing iron; b—Instrument sterilizing case. All the necessary instruments should be sterilized be- fore use. This may be done
. Laboratory work in bacteriology. Bacteriology. POST-MORTEM EXAMINATION OP INFECTED ANIMALS. 275 the common putrefactive bacteria from the intestinal canal. Consequently, in delayed examinations, impure cultures are apt to be met with. Moreover, an uncertainty will exist as to whether the secondary invasion occurred during life, or after death. Hence, in case the animal cannot be exam- ined at once, it should be placed in a suitable vessel and kept on Fig. 48. a—Searing iron; b—Instrument sterilizing case. All the necessary instruments should be sterilized be- fore use. This may be done by heating the instruments, di- rectly in the flame. Inasmuch as this procedure destroys the temper it should be avoided as much as possible. The best method of sterilizing- instruments, is to place these on a perforated tray which is then immersed in a copper or enameled iron box. The box should contain an almost sat- urated solution of borax (10 per cent.). The borax is to be preferred to sodium carbonate, since it prevents tarn- ishing as well as rusting of the instruments. The latter can be kept constantly in the borax solution. When it is desirable to sterilize these the box is placed on a Fletcher radial burner. In a few minutes the liquid will boil activ- ely. After 3 to 5 minutes of boiling, the flame can be turned off. This instrument sterilizer is shown in Fig. 48 &. A searing iron, also shown in Fig. 48, several sterile drawn out pipettes (Fig. 61), and several tubes of gelatin,. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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