. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and,
. A text-book of bacteriology; a practical treatise for students and practitioners of medicine. Bacteriology. METHODS USED IN CULTIVATION OF BACTERIA 145 The plates when fitted together are sterilized and thus form a closed cell which, if properly handled, may remain sterile indefinitely. The technique for maJdrtg a pour plate for the purpose of isolating bacteria from mixed culture is as follows: The actual "pouring" of plates is preceded by the preparation of usually three graded dilutions of the material to be examined. For this purpose three agar or gelatin tubes are melted and, in the case of the agar, are cooled to a temperature of about 42° C. in a water bath. A platinum loopful of the material to be examined is transferred to one of these tubes. The bacteria are then thoroughly distributed throughout the melted. EiG. 30.—Pouring Inoculated Mbdixjm into Petri Plate. gelatin or agar by alternately depressing and raising the plugged end of the tTibe, giving it a rotary motion at the same time. This thoroughly distributes the bacteria throughout the medium without allowing the formation of air-bubbles. Two loopfuls of this mixture are then trans- ferred to the second tube and a similar mixing process is repeated. This second tube contains the bacteria in much greater dilution than the first and the colonies which will form in the plate poured from this tube will be farther apart. A third dilution is then made by transferring five loopfuls of the mixture in the second tube to the third. This again is mixed as before. The contents of the tubes are then poured into three 11. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Hiss, Philip Hanson, 1868-1913; Zinsser, Hans, 1878-1940. joint author. New York and London, D. Appleton and Company
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Keywords: ., bookcentury1900, bookdecade1910, bookpublishernewyorkandlondonda
