. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. C. 15 25 15 25 25 Temperature (°C) FIGURE 4. Incorporation of 45Ca by spines of three specimens of 5". purpuratus (A-C) incubated in vitro for 24 hours at various temperatures after regeneration for four days in vivo in plain sea water following fracture. Each point represents the mean value of 10 spines, with vertical bars indicating ± Data for a 4th urchin are similar to those shown, except that only a slight increase in ^Ca incorporation occurred between 15° and 20° C. one another by pieces of glass rod of -J in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. C. 15 25 15 25 25 Temperature (°C) FIGURE 4. Incorporation of 45Ca by spines of three specimens of 5". purpuratus (A-C) incubated in vitro for 24 hours at various temperatures after regeneration for four days in vivo in plain sea water following fracture. Each point represents the mean value of 10 spines, with vertical bars indicating ± Data for a 4th urchin are similar to those shown, except that only a slight increase in ^Ca incorporation occurred between 15° and 20° C. one another by pieces of glass rod of -J inch diameter placed on the bottom of each dish. Temperatures varied by 1° C or less during the experiment. After incu- bation for 24 hours, all stubs from each animal were assayed for radioactivity by liquid scintillation. Figure 4 shows that incorporation of 45Ca by explants was directly proportional to temperature between ° and 20° C, at which a maximum occurred. At 26° C, little incorporation of the label took place, and tissue on all stubs appeared to be partially decomposed at the end of the experiment. Mean values for the tem- perature coefficient (Qio), and energy of activation (E) calculated over the intervals ° to °, ° to 15°, and 15° to 20° C, are , , ; and 26,052, 11,491, and 8662 calories per mole, respectively, with overall means be- tween ° and 20° C, of , and 15,504 calories per mole, respectively, for the four urchins studied. Activity of 45Ca measured in the second millimeter of the shaft of those stubs assayed was considered Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947;
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
