. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 132 BACTERIOLOGY. In the method recommended by Frankel a tube of gelatine is Hquefied, and inoculated. A gutta-percha stopper is substituted for the cotton-wool plug (Fig. 66). It is perforated by two holes, through which two tabes pass which are bent at a right angle. One tube only just passes through the stopper, the other reaches down to the bottom of the test-tube. The horizontal part of each tube has a narrow neck.
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. 132 BACTERIOLOGY. In the method recommended by Frankel a tube of gelatine is Hquefied, and inoculated. A gutta-percha stopper is substituted for the cotton-wool plug (Fig. 66). It is perforated by two holes, through which two tabes pass which are bent at a right angle. One tube only just passes through the stopper, the other reaches down to the bottom of the test-tube. The horizontal part of each tube has a narrow neck. The long tube has a plug of steril- ised cotton-wool, and is connected with a short piece of india-rubber tubing by which it can be connected with Kipp's apparatus. The hydrogen di'ives the air out of the liqviefied jellj^ and out of the test-tube, and after about half an hour â the horizontal tubes are sealed up, and the test-tube is made into a roll culture. Liborius employs a tube with a narrow neck and a lateral arm (Fig. 67). The tube is filled up to the height of the arm with either nutrient agar or a mixture of nutrient agar with 2 per cent, of grape- sugar. The hquefied jelly is inoculated in the usual way, and hydrogen passed through the lateral arm. When the air has been completely driven out, the tube is sealed up. To cultivate anaerobic organisms in broth, such as the tetanus bacillus, a flask is inoculated with the bacillus, and a stream of hydrogen is passed through the broth by means of a tube passing down to the bottom of the flask. The air in the flask escapes by a lateral arm which is bent â down at a right angle, and immersed in a capsule of mercury. When the air has been completely expelled the entrance tube is hermetically sealed, and the mercury in the capsule prevents any air from re-entering the flask by the lateral arm (Fig. 68).. Fig. 67.âAnaeeobic Cul- TUEE-TUBE (LiBOEIUS). Method of Fixing Cultures. The colonies in plate-cultivations and the grow
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