. Bacteria in relation to plant diseases. Bacteria; Plant diseases. 66 BACTERIA IN RELATION TO PLANT DISEASES. rod and 14 plates poured at 410 to 380 C, after inoculating very copiously—six 3 mm. loops in one instance. In other canes it was found alive at the end of a year. Tubes of bouillon inoculated November 9, 1903, and kept at room-temperatures were still cloudy on March 17 (129 days),but a transfer from one of them did not cloud bouillon. In beef-agar stabs kept in the refrigerator at io° to i5° cultures were alive at the end of 7 months 10 days. Under similar conditions another cul
. Bacteria in relation to plant diseases. Bacteria; Plant diseases. 66 BACTERIA IN RELATION TO PLANT DISEASES. rod and 14 plates poured at 410 to 380 C, after inoculating very copiously—six 3 mm. loops in one instance. In other canes it was found alive at the end of a year. Tubes of bouillon inoculated November 9, 1903, and kept at room-temperatures were still cloudy on March 17 (129 days),but a transfer from one of them did not cloud bouillon. In beef-agar stabs kept in the refrigerator at io° to i5° cultures were alive at the end of 7 months 10 days. Under similar conditions another culture was dead at the end of 7 months 18 days. Here the temperature may have been a few degrees higher, i. e.,\2° to 16° C. Another tube inoculated 14 days later and subject to the same conditions was alive at the end of 6 months 21 days. Two other tubes of the same lot were dead at the end of 6 months 12 days. Three other stab-cultures were dead at the end of 7 months 3 days. The organism was alive in cultures on potato- agarattheendof 7 months, but they were probably in the cold box. The organism was alive in 6 potato cultures at the end of 35 days (room- temperature about 25°C), but probably a large part were dead, as the transfers to fresh potato-cylinders grew slowly. On March 21, 1905, ten cultures on potato in the refrigerator at io° to 140 C. since October 6, 1904 (166 days) were tested for vitality, transfers being made to potato-agar (streaks). Two grew well; two feebly in small portions of the streak; and the rest did not grow. Of the two which grew well, one came from cane plant No. 40 and the other from cane plant No. 42. The organism has been found alive in milk cultures 54 days old; also in some 75 days old. Milk is a suitable culture medium for this organism. The organism is killed by sunlight (fig. 37).. Fig. 37.* RESUME OF SALIENT CHARACTERS. POSITIVE. Parasitic in sugar-cane, clogging the vascular bundles with a bright yellow slime and forming cavities
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