. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ISIT = BL IR = PREDATOR PREY LOCATION MIXER RECORDER Figure 1. Split-screen video camera arrangement for monitoring predator/prey interactions. at -1680 V (Latz et a!.. 1987). Cell samples were stim- ulated to exhaustion with a stirring rod consisting of a stainless steel shaft with three cross tines, coupled to a DC motor (Latz el a/., 1990) operating at a standard speed. Motor speed was measured with a magnetic pick-up mounted on the motor shaft and displayed on a Visi-tach digital ratemeter. Light emission was monitored f
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. ISIT = BL IR = PREDATOR PREY LOCATION MIXER RECORDER Figure 1. Split-screen video camera arrangement for monitoring predator/prey interactions. at -1680 V (Latz et a!.. 1987). Cell samples were stim- ulated to exhaustion with a stirring rod consisting of a stainless steel shaft with three cross tines, coupled to a DC motor (Latz el a/., 1990) operating at a standard speed. Motor speed was measured with a magnetic pick-up mounted on the motor shaft and displayed on a Visi-tach digital ratemeter. Light emission was monitored for 250 ms by ACE-MCS software operating with a channel dwell time of 5 ms. Previously unstimulated cells were run every 15 min for 3 h. Quantitative predation experiments with Sepia officinalis Twelve tests with single animals in 12-liter glass tanks were run concurrently in a darkroom. Six were controls, either with dinoflagellates absent or in photophase; and six were experimental tanks with dinoflagellates in sco- tophase. Tanks were separated by opaque dividers. Water temperature was maintained at 15°C. S. officinalis (av- erage mantle length = mm ± ; n = 50) were placed in individual tanks no later than 1 h before onset of the dark cycle to allow recovery after transfer. Dino- flagellates in final concentrations of 1. 2, 5. 10, 15. and 20 cells/ml were added 2 h after onset of scotophase. Be- cause cells tend to settle over time, concentrations indi- cated are for initial conditions. With care taken to min- imize bioluminescence. 10 mysids (carapace length: to ) were added simultaneously to all tanks. Preliminary experiments of up to 6 h were con- ducted to determine optimal time span and prey density. Results showed that Sepia of the ages used (2 to 4 months) were satiated after 3 h and never consumed more than 10 mysids during that time. To minimize disturbance and maintain dark adaptation, the Sepia were handled with the aid of an IR-light and IR-i
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
