. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 100 D. M. PACE AND BETTY LU FROST are scattered over the bottom of the dish. In Figure 1, a typical normal pelomyxa is compared with one grown in M alcohol solution. One of the most striking structural changes is found in the hyaline layer, which in normal organisms is a narrow clear area (except at the tips of pseudopods, where it is quite pronounced) found immediately beneath the plasmalemma. In pelomyxae exposed to M alcohol for 24 hours, the hyaline layer becomes greatly enlarged and well-defined in all parts of
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 100 D. M. PACE AND BETTY LU FROST are scattered over the bottom of the dish. In Figure 1, a typical normal pelomyxa is compared with one grown in M alcohol solution. One of the most striking structural changes is found in the hyaline layer, which in normal organisms is a narrow clear area (except at the tips of pseudopods, where it is quite pronounced) found immediately beneath the plasmalemma. In pelomyxae exposed to M alcohol for 24 hours, the hyaline layer becomes greatly enlarged and well-defined in all parts of the organism. The area becomes more noticeable from day to day, and appears to be most noticeable on about the fourth or fifth day of exposure. The increase in depth of the hyaline layer is noticed in all concen- trations from M and higher. Another noticeable change is in the decrease in size of the pelomyxae in the high alcohol concentrations. After 8 days in M alcohol, they are about 1/10 their original size. This is illustrated well in the figure. A B FIGURE 1. Camera lucida sketches of (A) a typical pelomyxa from a well-balanced cul- ture solution without alcohol, and (B) one from the same solution containing alcohol in M concentration. F. V., food vacuole; C. V., contractile vacuole; H., hyaline area. The rate of digestion of food organisms is also retarded in high concentrations of alcohol. In the controls and in the M to M alcohol solutions, most of the food vacuoles contain only fragments of paramecia after 24 hours while in higher concentrations rather large fragments are found even after 5 days. In the M alcohol solution the food vacuoles are carried over from the time of in- gestion before exposure to alcohol; no paramecia are ingested by the pelomyxae after the latter are exposed to this concentration. 3. Effect of alcohol on respiration in Pelomyxa The Barcroft-Warburg respirometer was used to ascertain oxygen consumption. This wa
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
