. Biological structure and function; proceedings. Biochemistry; Cytology. 244 PHILIP SIEKEVITZ TABLE III Effect of Spermine and of Mg + + on Release of RNA and Protein from RNP Particles Incubation conditions are given in the text. Exp. No. Additions Left in RNP particles after spinning incubated particles for 90 min. at 105 000 x g RNA 4 None Spermine (10"* m) Spermine (10^ m) plus Mg + + (io~^ m) Protein RNA/ Protein I None 229 341 0-67 Spermine (Io"* m) 209 278 0-75 Spermine (lO"* m) plus Mg + +(lo"^ m) 273 319 0-85 2 Spermine (10 * m) Spermine (10 * m) plus Mg + + (10^
. Biological structure and function; proceedings. Biochemistry; Cytology. 244 PHILIP SIEKEVITZ TABLE III Effect of Spermine and of Mg + + on Release of RNA and Protein from RNP Particles Incubation conditions are given in the text. Exp. No. Additions Left in RNP particles after spinning incubated particles for 90 min. at 105 000 x g RNA 4 None Spermine (10"* m) Spermine (10^ m) plus Mg + + (io~^ m) Protein RNA/ Protein I None 229 341 0-67 Spermine (Io"* m) 209 278 0-75 Spermine (lO"* m) plus Mg + +(lo"^ m) 273 319 0-85 2 Spermine (10 * m) Spermine (10 * m) plus Mg + + (10^ m) 188 246 272 275 0-69 0-89 3 Spermine (10"* m) Spermine (10"* m) plus Mg + + (10"^ m) 116 156 250 271 0-47 0-58 211 315 0-67 154 282 0-55 176 287 062. 0 6 12 18 24 30 36 42 48 54 60 GTP (xIO'^'m) Fig. I. Effect of GTP concentration on the bound radioactive spermine, on the RNA, and on the RNA/protein ratio of the RNP particles. Methods are given in the text. addition of Mg + + along with the spermine results in a very weak Mg + +- spermine complex and hence has no effect on the salts formed between the spermine and the phosphate groups. Since the spermine would appear. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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