. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 M. ENZIEN ET AL. Figure 4. Photomicrographs of live Paratetramitus jugosus. A. Amoeba with visible nucleus and sev- eral small spherical and irregular-shaped bodies. Cyst in bottom left corner. Small spherical bodies frequently found in cultures. D-H. Sequential photos showing movement of small amoeba (arrow). The small amoeba closely resembles many other small irregular objects in our cultures. (Compare 4C with 4G) Bar scale = 10 jim. Coverslips were placed on blotting paper, sample-side up, and flooded with approx
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 116 M. ENZIEN ET AL. Figure 4. Photomicrographs of live Paratetramitus jugosus. A. Amoeba with visible nucleus and sev- eral small spherical and irregular-shaped bodies. Cyst in bottom left corner. Small spherical bodies frequently found in cultures. D-H. Sequential photos showing movement of small amoeba (arrow). The small amoeba closely resembles many other small irregular objects in our cultures. (Compare 4C with 4G) Bar scale = 10 jim. Coverslips were placed on blotting paper, sample-side up, and flooded with approximately 200 ^1 of stain solution. A clean glass slide placed over the coverslip was turned over and allowed to stand for one to three hours in the dark. The slide was then blotted dry and sealed around the edges with clear nail polish. Some slide preparations were treated with pancreatic RNase before staining to re- move background binding of DAPI to RNA (Coleman et a/.. 1981). Samples treated with bovine DNase I (Coleman el a/., 1981) allowed distinction of stained DNA from. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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