. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. con 40 pm Figure 4. Light micrographs of l-/jm sections of accessory nida- mental (AN) gland of S7-day-old juvenile. (A) Gland (an) and associated ink sac (is). Magnification bar = 100 ^m. (B) Early tissues of AN gland (an), including the basal connective tissues (con). Magnification bar = 40 nm. A \ con an li -Jfoo (jm. Figure 5. Light micrographs of 1-^m sections ot epithelial cells ot the accessory nidamental (AN) gland in lOO-day-old juvenile. (A) View highlighting gland attachment to the outer muscular wall (omw) of the
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. con 40 pm Figure 4. Light micrographs of l-/jm sections of accessory nida- mental (AN) gland of S7-day-old juvenile. (A) Gland (an) and associated ink sac (is). Magnification bar = 100 ^m. (B) Early tissues of AN gland (an), including the basal connective tissues (con). Magnification bar = 40 nm. A \ con an li -Jfoo (jm. Figure 5. Light micrographs of 1-^m sections ot epithelial cells ot the accessory nidamental (AN) gland in lOO-day-old juvenile. (A) View highlighting gland attachment to the outer muscular wall (omw) of the ink sac. Magnification bar = 100/vm. (B) Cells of AN gland (an) or- ganizing into tubules atop differentiating connective tissues (con). Mag- nification bar = 40 ^m. No bacteria were evident on the surface of the 87-day AN gland epithelial cells. Between each epithelial cell, typical cell junction complexes were seen and a relatively undifferentiated connective tissue layer was in contact with the basal surface of the cells (Fig. 48). By 100 days, differentiation of connective tissue pushed the epithelial cell layer towards the mantle cavity. A second layer of epithelial cells was observed, and cilia were occasionally seen on the apical surface (Fig. 5A, B). No bacteria were yet observed in association with the tissue of the AN gland. In the 129-day-old juvenile, the cell layers of the AN gland were deeply invaginated, forming the primordial tubules (Fig. 6A). In many of these tubules, the end of the structure remained open to the mantle cavity ( Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Cla
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