. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. PNEUMONIA. 235 (a) Stain by the method of Gram, and after-stain with eosin. ' , (6) Treat with acetic acid, then stain with gentian-violet or Bismarck- brown. Examine in distilled water, or dry and preserve in Canada balsam. (c) Float them on weak solutions of the aniline dyes twenty-four hours ; differentiation between coccus and capsule is thus obtained. (d) Stain with osmic acid ; the contour of the capsules is broug
. A text-book of bacteriology, including the etiology and prevention of infective diseases and a short account of yeasts, and moulds, haematazoa, and psorosperms. Bacteriology. PNEUMONIA. 235 (a) Stain by the method of Gram, and after-stain with eosin. ' , (6) Treat with acetic acid, then stain with gentian-violet or Bismarck- brown. Examine in distilled water, or dry and preserve in Canada balsam. (c) Float them on weak solutions of the aniline dyes twenty-four hours ; differentiation between coccus and capsule is thus obtained. (d) Stain with osmic acid ; the contour of the capsules is brought i^ t Fig. 115.—Capsule-coooi from Pneumonia, x 1500 (Baumgabtbn). Sections of pneumonic lung should be stained by— (a) Method of Gram. (J) Method of Friedlander. This method is employed to demonstrate the capsules in tissue sections. It consists in placing the sections twenty- four hours in the following solution :— Fuchsine . . 1 Distilled water . 100 Alcohol ... . 5 Glacial acetic acid . ... 2 They are then rinsed with alcohol, transferred for a couple of minutes to a 2 per cent, solution of acetic acid, and treated with alcohol and oil of cloves in the usual way, and preserved in Canada balsam. Sternberg's micrococcus was first found in the blood of rabbits inoculated with saliva. Three months afterwards, Pasteur encoun- tered the same organism in rabbits inoculated with the blood of a child suffering from rabies. The same orgariism in 1883 was found by Talamon in pneumonic sputum. It was identified by Sternberg. Two years afterwards further observations were made by Frankel, Gamaleia, and others. It has also been found in purulent meningitis by Netter, and by Monti in cerebro-spinal meningitis, by Weichsel- baum in ulcerative endocarditis, and by others in acute abscess of the middle ear, and in purulent inflammation of the joints following Please note that these images are extracted from scanned page images that may have been digitally enhanced f
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