. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SETTLEMENT INDUCTION BY HISTAMINH 167 100 -i c 75- 50- 8. 25-. o o o o o o in p* o treatment Figure 3. The settlement (%) of larvae of Holopneustes fiiirpiinisci'm, after I h incubation with the polar extract of Delisea pulchra (PE) and cation-exchange fractions (F) of the PE. The different test concentrations of each treatment are shown in brackets (/xg • mP1): note the lower concen- trations for F5 and the procedural control (CF5). Sterile seawater (SSW) was used as the negative control (n = 10). Gas chromatography—mass sp
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. SETTLEMENT INDUCTION BY HISTAMINH 167 100 -i c 75- 50- 8. 25-. o o o o o o in p* o treatment Figure 3. The settlement (%) of larvae of Holopneustes fiiirpiinisci'm, after I h incubation with the polar extract of Delisea pulchra (PE) and cation-exchange fractions (F) of the PE. The different test concentrations of each treatment are shown in brackets (/xg • mP1): note the lower concen- trations for F5 and the procedural control (CF5). Sterile seawater (SSW) was used as the negative control (n = 10). Gas chromatography—mass spectrometry. The identity of putative histamine (F5) isolated from D. pulchra was con- firmed using GC-MS. The retention times (rt) of the hep- tafluorobutyrlacyl derivative of putative histamine (rt = ± mean ± SD. /; = 5) and synthetic hista- mine (rt = ± mean ± SD. n = 5) were nearly identical, suggesting that they were the same compound. The electron-impact ion spectra of both derivatized com- pounds displayed the same major fragment ions (m/-—54. 69. 81, 94. 138. 169. 226. 307. 349) and overall fragmen- tation pattern, confirming that they were the same com- pound. The electron-impact ion spectra for derivatized his- tamine matched that reported in the literature (Barancin ct a!.. 1998). Matrix-assisted laser desorptlon/ionization—time-of-flight mass spectnunetry. The elemental formula of putative his- tamine isolated from D. pulchra was confirmed by accurate mass measurements using MALDI-TOF MS. The measured accurate mass of the putative protonated histamine molec- ular ion [M + H] + was ± (n = 10, mean ± SD), and the measured mass for synthetic histamine was ± (n = 10. mean ± SD). The measured masses of the two samples were different by only ppm. These values were different from the calculated monoiso- topic mass for protonated histamine (—elemen- tal formula C_SHH)N3) by only 15 p
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
