. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 520 A. K. PAR I'ART. \V. R. AMBERSON, AND D. R. STEWART In theM- hemoglobin determinations it is not necessary to know the relation existing between the intensity of the pyrometer lamp and the current passing through it, since all readings are evaluated by reference t a calibrated curve made with hemoglobin solutions of known con- centration. An essential feature in securing high sensitivity is the use of a green glass filter (A) which is placed at the eyepiece of the telescope. The transmission characteristics of this filte
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 520 A. K. PAR I'ART. \V. R. AMBERSON, AND D. R. STEWART In theM- hemoglobin determinations it is not necessary to know the relation existing between the intensity of the pyrometer lamp and the current passing through it, since all readings are evaluated by reference t a calibrated curve made with hemoglobin solutions of known con- centration. An essential feature in securing high sensitivity is the use of a green glass filter (A) which is placed at the eyepiece of the telescope. The transmission characteristics of this filter presented in Fig. 2 were ob- tained spectrophotometrically through the kindness of Dr. D. L. Drab- kin. It will be noticed that the filter has its maximum transmission in 600 500 450 0 550 a Wave Length I1"in. 2. Transmission curve for the green filter. the region of maximum absorption bv hemoglobin, whether the latter is in the oxygenated or reduced condition, or in the form of methemo- globin or acid bematin. A 20 cm. polariscope tube ( /' ). containing the hemoglobin solution, is placed on a rigid stand (/•") between the two lamps. The usual plate- glass disc covers the end of the tube facing the pyrometer lamp, while at the other end there is a glass disc ground on one surface. This latter disc serves to diffuse the light from the background filament. Both of these discs must always be replaced in their original position as rotation will vary their transmission. The readings ha\e been taken with the background lamp set and stabili/ed at amperes. \Vith the hemoglobin solution in place, the i •* amperage- of the pyrometer filament is varied until the portion selected for observation just disappears, proceeding in every case from dark to light. The milliamperagc of the pyrometer lamp is recorded and the. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearanc
Size: 1909px × 1308px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
