. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 502 M. D. OHMAN ET AL >~ T 0 16 b CO 03 > - O g O 01 004' A. Initial Strombidium density (cells ml) Figure 2. (A) Relation between the gut contents of first feeding anchovy larvae as determined from immunochemical dot blots (x ± 95%) and the concentration of ciliate prey. Optical density readings have been corrected for the reaction blank from starved larvae. (B) As in part A, with gut contents expressed as ingested ciliate protein content larva ' from the relation in Figure 1. Anchovy larvae were raised from eggs coll
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 502 M. D. OHMAN ET AL >~ T 0 16 b CO 03 > - O g O 01 004' A. Initial Strombidium density (cells ml) Figure 2. (A) Relation between the gut contents of first feeding anchovy larvae as determined from immunochemical dot blots (x ± 95%) and the concentration of ciliate prey. Optical density readings have been corrected for the reaction blank from starved larvae. (B) As in part A, with gut contents expressed as ingested ciliate protein content larva ' from the relation in Figure 1. Anchovy larvae were raised from eggs collected in surface waters off La Jolla, California, and maintained in glass-fiber (GF/F) filtered seawater at 15°C under fluorescent lamps (12 ^Ein m"2 s ')ona 12:12 light: dark cycle. Containers for predation experiments were black polypropylene beakers containing 800 ml of filtered seawater, covered with Mylar lids. Only first-feeding larvae (3-4 days post-hatch, mm standard length) were used. Ciliates (Strombidium sp. clone AH) were grown on a monoxenic diet of the bacterium \'ibrio natriegens (6). The dimensions of stationary phase Strombidium sp. are 25 x 30 ^m (width * height; preserved in 2% acid Lugols and thus reflecting cell shrinkage), cell biovolume 12,500 ^m3 (live volume), ng protein ciliate"', and ng C ciliate"' (6). All Strombidium used in predation experiments were in stationary growth phase. Larvae were incubated for 6-8 h with Strombidium sp. Seven to 13 individual larvae were analyzed per treatment. After predation experiments, larvae were quickly frozen in liquid N:, then transferred to a —80°C freezer. Control first-feeding larvae (maintained continuously in filtered seawater) were treated in the same manner. For all immunoassays, guts of thawed larval anchovy were individually dissected onto a glass microscope slide. The gut contents were teased into 2-4 n\ of PBS buffer, then blotted onto pre-washed nitrocellulose. A standar
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
