. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Total RNA dot blots of Dreissena polymorpha foot (F). adductor muscle (A), mantle (M). and gill (G) tissue hybridized to a digoxigenin-labeled RNA probe specific to Dpfpl. The probe was hybridized to 1 of total RNA from each tissue. as a byssal structural protein. Northern blots of foot tissue mRNA indicated that Dpfpl transcripts range in size from 1200 b to 1500 b. suggesting the presence of size variants (Fig. 2). Dpfpl cDNA sequence In Figure 3 the aligned nucleotide sequence data ob- tained from 5' RACE.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Figure 1. Total RNA dot blots of Dreissena polymorpha foot (F). adductor muscle (A), mantle (M). and gill (G) tissue hybridized to a digoxigenin-labeled RNA probe specific to Dpfpl. The probe was hybridized to 1 of total RNA from each tissue. as a byssal structural protein. Northern blots of foot tissue mRNA indicated that Dpfpl transcripts range in size from 1200 b to 1500 b. suggesting the presence of size variants (Fig. 2). Dpfpl cDNA sequence In Figure 3 the aligned nucleotide sequence data ob- tained from 5' RACE. RT-PCR with degenerate oligonu- cleotide primers, and from the largest cDNA clone iso- lated are presented. Each sequence differs slightly from the other, and therefore the consensus sequence generated from this alignment does not represent any single Dpfpl sequence. It is likely that differences in the data sets reflect the existence of Dpfpl variants rather than errors introduced during amplification, because each set of PCR < kb. < Figure 2. Northern blot of Dreissena polymorpha foot tissue mRNA hybridized to a digoxigenin-labeled RNA probe specific to Dpfpl; 3 fjg of foot tissue mRNA was used. sequence data was determined from at least two indepen- dently amplified samples. The combined transcript is 1481 bp in length and contains an open reading frame of 1332 bp coding for a protein of 443 amino acids. Included in the transcript is a start codon at nucleotide position 36 and two overlapping canonical polyadenylation signals (Kozak. 1986) at nucleotide positions 1464 and 1468. The calculated molecular weight of the deduced primary sequence is 49 kDa. with a predicted isoelectric point of The first 19 amino acids code for a putative signal peptide that conforms to the rule of von Heijne (1985). Computer-based modeling of signal peptide cleavage (Nielsen et al., 1997) correctly predicts cleavage of the signal peptide preceding the previously determined N-
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