. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Staining Paraffin Sections 67 Staining Chart II. The same slide can be repeatedly destained or restained in the counterstain until exactly the desired effect is obtained. The hematoxylin is not affected during this manijjulation. STAINING CHART II Hemalum With "General" Counterstain Pre-Staining Operations and Intervals as in Chart I Hemalum to correct intensity i 30% alcohol i 50% alcohol i 70% alcohol i 95% al
. Botanical microtechnique. Botany -- Anatomy; Botany -- Morphology; Microscopy -- Technique; Microscopes -- Technique; Botany; Plants -- anatomy & histology; Microscopy. Staining Paraffin Sections 67 Staining Chart II. The same slide can be repeatedly destained or restained in the counterstain until exactly the desired effect is obtained. The hematoxylin is not affected during this manijjulation. STAINING CHART II Hemalum With "General" Counterstain Pre-Staining Operations and Intervals as in Chart I Hemalum to correct intensity i 30% alcohol i 50% alcohol i 70% alcohol i 95% alcohol erythrosin (see text) >. Other common counterstains used with the above hematoxylins, are orange G, gold orange, eosin, fast green, and light green. The underlying principle for applying other counterstains is the same as for erythrosin. Counterstains may also be dissolved in clove oil and applied after the last dehydrating step, omitting carbol-xylene because clove oil is an excellent clearing agent. Counterstains may also be dissolved in water, 50% to absolute alcohol, or Cellosolve and introduced into the series at the corresponding point of dehydration. HEMALUM AND SAFRANIN After acceptable results have been obtained with the foregoing single stain and the double stain, undertake the mastery of a double stain having two selective components. One component of the next double stain to be discussed is a self-mordanting hematoxylin; the second component is safranin, which is highly selective for chromo- somes, lignin, cutin, and in some cases for hemicellulose. An important feature of this combination is that the hemalum is applied to the desired intensity and remains fixed throughout subsequent processing, whereas the safranin is applied until the material is strongly overstained and then differentialh Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of th
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