. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . dinary aniline dyes and are readilydecolorized by Grams solution. A smear from a culture shows charac-teristic irregularity in staining, some of the cocci taking the counter- 278 MENINGOCOCCUS stain poorly and some staining deeply. The positive cocci described by Jaeger and others were probably contaminating organisms. The cells show no definite —They grow between 25° and 38° C, best at about 35° C. They are most easily isolated on 2 per cent, glucose ascitic agar neutral to phe
. Pathogenic microörganisms; a practical manual for students, physicians, and health officers . dinary aniline dyes and are readilydecolorized by Grams solution. A smear from a culture shows charac-teristic irregularity in staining, some of the cocci taking the counter- 278 MENINGOCOCCUS stain poorly and some staining deeply. The positive cocci described by Jaeger and others were probably contaminating organisms. The cells show no definite —They grow between 25° and 38° C, best at about 35° C. They are most easily isolated on 2 per cent, glucose ascitic agar neutral to phenolphthalein. They arekept well on semisolid liver medium recommendedby Dopter (p. 105) is an excellentone for stock transplants. Thereaction of the media is veryimportant. A cultiu-e can rarelybe isolated on plain nutrient strains of meningo-cocci vary somewhat in the easewith which they may be culti-vated, their ability to fermentcarbohydrates, virulence for ani-mals, agglutinability, degree ofdigestibility in leukocytes, andpower of resistance to Fig. 109.—Diplococous intracellularis men^ingitidis in pus cells. X 1100 diameters; After having been isolated for some time, a tolerably good growth developsat the end of forty-eight hours in the incubator. On semitransparent media(glucose-ascitic agar) the colonies may be seen as a flat layer, each about i inchin diameter, grayish white in color, finely granular, rather viscid, and non-con-fluent unless very close together. On Lofflers blood serum the growth formsround, whitish, shining, viscid-looking colonies, with smooth and sharplydefined outlines; these may attain diameters of ^V to | inch in twenty-fourhours. The colonies tend to become confluent and do not liquefy the the spinal fluid in acute cases, where the organisms are apt to be moreabundant, a great many minute colonies may develop instead of a few largerones. On agar plates the deep-lying colonies are almost i
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