A manual of the modern theory and technique of surgical asepsis . —Staining the bacillus tuberculosis. No scientific method of isolating and cultivat-ing a distinct species from a mixture of thesedifferent microbes was known until Robert Kochdiscovered the mode of disseminating a mixtureof them over a large surface in order to favorthe development of the various species. Thenthe different forms of vegetation could be recog-nized with the naked eye as spots or turfs of apeculiar shape, color, growing species, etc. On an 3° SURGICAL ASEPSIS. artificially prepared soil, especially on gelatin oron
A manual of the modern theory and technique of surgical asepsis . —Staining the bacillus tuberculosis. No scientific method of isolating and cultivat-ing a distinct species from a mixture of thesedifferent microbes was known until Robert Kochdiscovered the mode of disseminating a mixtureof them over a large surface in order to favorthe development of the various species. Thenthe different forms of vegetation could be recog-nized with the naked eye as spots or turfs of apeculiar shape, color, growing species, etc. On an 3° SURGICAL ASEPSIS. artificially prepared soil, especially on gelatin oron agar, pure cultures can readily be of them are characterized by their capacityto liquefy the gelatin, while others form whitedry heaps or white mucilaginous drops, or formcolonies of a yellow, green, or red color. A very important aid in distinguishing thesedifferent forms under the microscope is theirstaining (Fig. 9) with certain coloring matters, es-pecially aniline dyes, and the so-called Abbesillumination, a method which allows a distinct. Fig. 10.—Making gelatin cultures. ocular perception of the stained microbes. Kochsinvestigations are based upon the necessity ofcultivating these species pure ; that is, they mustbe free from all accidental admixtures. In orderto obtain this purity in cultivation a small quan-tity of the substance containing the microbesis implanted upon some suitable liquid soil, meator agar-agar gelatin, to which peptone is added,being generally preferred (Fig. 10). The soil must INFLUENCE OF MICROBES. 31 first be freed from all foreign microbes—that is,it must be sterilized—and then the cultivation mustbe conducted in an incubator (Fig. 11). From
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectsurgery, bookyear1895
