. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Protein Stain Autoradiograph -122 kD- E a. 500- 80 o a 1000 (M CM 1500- ° 2000- O 2500 Q. 3000- LON LON Cut Intact LON LON Cut Intact. -100 90 a_ a (M CM Figure 3. The 122 kD protein is phosphorylated in vivo in the Limuliis lateral eye in response to activa- tion of the efferent neurons by the circadian clock. This figure shows the results of a single assay (Table IA; animal 1, assay 1). Early in the day, the LON was cut just anterior to one of the eyes to block efferent input to that eye. Input to the other eye was left in
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Protein Stain Autoradiograph -122 kD- E a. 500- 80 o a 1000 (M CM 1500- ° 2000- O 2500 Q. 3000- LON LON Cut Intact LON LON Cut Intact. -100 90 a_ a (M CM Figure 3. The 122 kD protein is phosphorylated in vivo in the Limuliis lateral eye in response to activa- tion of the efferent neurons by the circadian clock. This figure shows the results of a single assay (Table IA; animal 1, assay 1). Early in the day, the LON was cut just anterior to one of the eyes to block efferent input to that eye. Input to the other eye was left intact. The animal was then placed into the dark in a standard apparatus for recording the ERG of the intact eye (Barlow, 1983). In the record shown, the ERG amplitudes were recorded every 10 min. Before 16:20, the small ERG amplitudes were partially obscured by voltage noise, probably arising from muscle contractions. After 16:20, the ERG amplitudes were clearly visible, and they began to increase after 17:10 as a result of efferent nerve activity. The ERG amplitudes (light sensitivity) stabilized at an elevated level by 19:00. At 22:00, both eyes were removed from the animal and immediately immersed in liquid N:. Each eye was then briefly placed in ice cold saline so that the cornea could be removed, the tissue proteins were solubilized, and the amount of endogenous phosphorylation associated with the 122 kD protein from each eye was determined by the back phosphorylation procedure. The lower portion of this figure shows (left to right): the pattern of proteins visualized by silver stain, (aliquots of solubilized preparations of both eyes contained about the same amount of 122 kD protein); an autoradiograph of a nitrocellulose blot containing larger (4 • ) aliquots of these samples, subjected to the back phosphorylation procedure; and the amount of 3:PO4 associated with the 122 kD protein from both eyes. The relative amount of endogenous phosphorylation in response to effere
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
