. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Kinesin. •NF200 Kinesm • <- NF60 4—Tubulin 4— Actin Ic It 2c 2t Ic It 2c 2t Figure I. Effects ofaxotomy on axonal transport, structure and proteins. Fluorescent tuhulin injected into an axon moved anterogradely (to the right) away from the injection site (arrowhead in A). Tubuiin injected into tin axon that had been separated from its cell bodies 2 d prior showed no anterograde transport (B). This tubulin just diffused in both directions around tile injection site (arrowhead in B). Darkfield light scattering was unaffecte
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. Kinesin. •NF200 Kinesm • <- NF60 4—Tubulin 4— Actin Ic It 2c 2t Ic It 2c 2t Figure I. Effects ofaxotomy on axonal transport, structure and proteins. Fluorescent tuhulin injected into an axon moved anterogradely (to the right) away from the injection site (arrowhead in A). Tubuiin injected into tin axon that had been separated from its cell bodies 2 d prior showed no anterograde transport (B). This tubulin just diffused in both directions around tile injection site (arrowhead in B). Darkfield light scattering was unaffected by axotomy. Light scattering was the same in control (C) as it was in transected axons (D). Higher resolution video enhanced differential interference microscopy also showed no difference between control (El and paired transected (F) axons. Mitochondria (arrows in E and F) and smaller organelles (arrowheads) were present in control and transected axons. Scale bars: 100 /jjnforA and B. 50 fjjnfor C and D. and I fjjn for E and F. Solubilized axonal proteins (G)from equal lengths of axon from the control (Ic) and the transected side (It) of a squid that had been axotomized 2 d earlier showed no change in protein-staining intensity. Paired axons from a smaller squid (2) with shorter axons had proportionately less protein than the larger squid (1). but there was no significant difference in protein levels between the control side (2c) and the transected side (2t). Similarly, axotomy had no effect on total immunochemically measured kinesin levels (H) in the paired axons from these same nvo squids. (Ic vs. It and 2c vs. 2ti. The present experiment suggests that some unidentified factor essential to axonal tubulin transport is lost or inhibited 2 days after axotomy. This inhibition is not due to the depletion of conven- tional kinesin. It could be due to the inactivation of conventional kinesin or to the inactivation or depletion of some other motor or factor essential to axo
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
