. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 WILLIAM R. HARVEY be observed that gas continued to disappear long after tbe calculated 325 /A. of oxygen would have been used up. The substantial anaerobic capacity of the pupae (Harvey and Williams, 1958a; 1958b) enabled the continuation of the ex- periment for four days. At that time the function was tending toward a plateau somewhat short of the 975 pi. (325 of oxygen plus 650 fA. of carbon monoxide) predicted for removal of the oxygen solely by carbon monoxide-oxidation. These results, together with the similar
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 16 WILLIAM R. HARVEY be observed that gas continued to disappear long after tbe calculated 325 /A. of oxygen would have been used up. The substantial anaerobic capacity of the pupae (Harvey and Williams, 1958a; 1958b) enabled the continuation of the ex- periment for four days. At that time the function was tending toward a plateau somewhat short of the 975 pi. (325 of oxygen plus 650 fA. of carbon monoxide) predicted for removal of the oxygen solely by carbon monoxide-oxidation. These results, together with the similar results obtained with the other five preparations, 500h. ANIMAL PRESENT ANIMAL ABSENT I I 0 20 30 HOURS 40 50 FIGURE 3. The time-course of the disappearance of carbon monoxide from Warburg vessels containing alkali and carbon monoxide, but no oxygen. clearly demonstrate that carbon monoxide is removed from the system by the living pupa. Since this does not occur in the absence of oxygen (see Section 2) one may infer that carbon monoxide is being combusted. 2. Correction for tlic non-oxidative removal of carbon monoxide The manometric data just described were corrected for the reaction of carbon monoxide with alkali (Warburg, 1927) in the following way. Four 45-ml. War- burg vessels were prepared as in the above experiment but no animals were added. Three were flushed with 20 volumes of carbon monoxide that had been washed through potassium hydroxide and lead acetate (Method 6). The fourth vessel contained air and served as a thermobarometer. The uptake of carbon monoxide from each vessel, plotted as a function of time in Figure 3, amounted to ± () Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Ho
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
