The Journal of biological chemistry . all the blood had left the saturator both cocks wereclosed and the rubber tubings were clamped. The saturator andthe bottle were then removed from the bath and rubber stopper was removed from the bottle and the paraffinoil was removed as completely as possible by means of a paraffin at a temperature of 50° was poured into the bottle,in sufficient amount to form a layer about 1 inch thick on top of theblood. When the paraffin had solidified the blood was centrifuged. To remove the serum a hole about 1 cm. in diameter was bore
The Journal of biological chemistry . all the blood had left the saturator both cocks wereclosed and the rubber tubings were clamped. The saturator andthe bottle were then removed from the bath and rubber stopper was removed from the bottle and the paraffinoil was removed as completely as possible by means of a paraffin at a temperature of 50° was poured into the bottle,in sufficient amount to form a layer about 1 inch thick on top of theblood. When the paraffin had solidified the blood was centrifuged. To remove the serum a hole about 1 cm. in diameter was boredthrough the paraffin by means of a heated cork borer. About15 cc. of the serum for CO2 determination were transferred overmercury to a receiver by means of a bent capillary tube,^ arrangedas shown in Fig. 116. As receivers in these experiments weemployed the tubes forming the lower chambers of the 2-cham- 8 See Austin, Cullen, Hastings, McLean, Peters, and Van Slyke (5),p. 132. 816 Electrolyte and Water Distribution To ^ levelling. Blood
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Keywords: ., bookcentury1900, bookdecade1900, booksubjectbiochem, bookyear1905
