. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NUCLEATED ASSEMBLY OF DTAF-TUBULIN 361. FIGURE 1. Polyacrylamide gel electrophoresis of the fluorescently modified proteins. The unstained gels were photographed under UV illumination to visualize the fluorescence (a and b); identical gels were then stained for protein with Coomassie Brilliant Blue (c and d). Lanes a and c are phosphocellulose purified DTAF labeled tubulin dimers; lanes b and d are DTAF labeled MAPs prepared according to Vallee (1982) (see Methods). Both the a- and /3-subunits of tubulin are distinctly fluor
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. NUCLEATED ASSEMBLY OF DTAF-TUBULIN 361. FIGURE 1. Polyacrylamide gel electrophoresis of the fluorescently modified proteins. The unstained gels were photographed under UV illumination to visualize the fluorescence (a and b); identical gels were then stained for protein with Coomassie Brilliant Blue (c and d). Lanes a and c are phosphocellulose purified DTAF labeled tubulin dimers; lanes b and d are DTAF labeled MAPs prepared according to Vallee (1982) (see Methods). Both the a- and /3-subunits of tubulin are distinctly fluorescent as are the high molecular weight MAPs. The stoichiometry of labeling was determined as described by Keith et ai, (1981) using an extinction coefficient of X 105 for DTAF and a molecular weight of 110,000 for the tubulin dimer. At a final stoichiometry of (moles of flu- orochrome bound per mole of protein) the proteins retained their native characteristics and were distinctly fluorescent. Interaction of the fluorescent protein with the isolated mitotic apparatus To determine whether the fluorescent protein retained the ability to assemble in association with microtubule organizing centers (MTOCs, Pickett-Heaps, 1969) in vitro, the fluorescently modified protein was incubated with meiotic spindles isolated from oocytes of the surf clam, Spisula solidissima. Incubation of isolated spindles with fluorescent MT protein maintained or augmented the birefringence of the isolated spindles in a temperature and concentration dependent fashion (Fig. 6). For example, when isolated Spisula meiotic apparatuses (MAs) (Fig. 6a) were resuspended in po-. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annu
Size: 1372px × 1821px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
