. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 320 L. H. CHAPPELL, C. ARME, AND C. P. READ palmitate uptake are shown in Figure 5. Varying the concentrations from mM to mM desoxycholate did not alter palmitate uptake, but concentrations of bile salt in excess of mM brought about a twofold increase in the transport of palmitate. The effects of increasing the concentration of desoxycholate above mM could not be examined due to the formation of an insoluble precipitate. The use of a 10 mM. palmitate stock solution In order to extend the range of concentrat
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 320 L. H. CHAPPELL, C. ARME, AND C. P. READ palmitate uptake are shown in Figure 5. Varying the concentrations from mM to mM desoxycholate did not alter palmitate uptake, but concentrations of bile salt in excess of mM brought about a twofold increase in the transport of palmitate. The effects of increasing the concentration of desoxycholate above mM could not be examined due to the formation of an insoluble precipitate. The use of a 10 mM. palmitate stock solution In order to extend the range of concentrations over which the uptake of 14C-palmitate could be investigated, a 10 mM palmitate stock solution was made up (previous data refer to dilutions of a 2 mM palmitate stock). The uninhibited uptake of palmitate diluted from the stronger stock solution is shown in Figure 1, curve b. It is evident that, at all concentrations, the palmitate uptake from a diluted 10 mM stock is greater than that from a diluted 2 mM stock (curve a). The 10 mM curve also showed non-linearity and, hence, a Kt ( mM) and Vmax ( /*Moles/g/2 min) could be determined. •6 •4. •2 -4 -6 -8 mM palmitate FIGURE 4. The uptake of 14C-palmitate at pH (Curve b), pH (Curve c) and pH (Curve a) as a function of palmitate concentration. Each point is the mean of 8 replicates. Because of the differences demonstrated between uptake from dilutions of stocks of different strengths, the effects of various higher fatty acids were investigated using diluted 10 mM stock palmitate. The results of these experiments are shown in Table III. At concentrations of mM, all fatty acids investigated, with the exception of heptadecanoate, were more effective inhibitors of palmitate uptake when dilutions from 10 mM stock solutions were used than was the case with dilutions from 2 mM stock solutions. Furthermore, oleate, at a concentration previously shown to be stimulatory (, at the same concentration as pal
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