. Biology of the laboratory mouse. Mice as laboratory animals; Mice; Animals, Laboratory; Mice. 68 BIOLOGY OF THE LABORATORY MOUSE smearing with a spatula tends to disturb the regularity of the cycle (113, 133). The addition of a small amount of methylene blue to the water used gives a very satisfactory stain. With this stain smears can be examined at once without waiting for the water to dry. Three types of cells are found in vaginal smears, i. Leukocytes (Fig. 34A). In unstained preparations these appear at first as small, round, highly refractive cells, but they swell rapidly in water with
. Biology of the laboratory mouse. Mice as laboratory animals; Mice; Animals, Laboratory; Mice. 68 BIOLOGY OF THE LABORATORY MOUSE smearing with a spatula tends to disturb the regularity of the cycle (113, 133). The addition of a small amount of methylene blue to the water used gives a very satisfactory stain. With this stain smears can be examined at once without waiting for the water to dry. Three types of cells are found in vaginal smears, i. Leukocytes (Fig. 34A). In unstained preparations these appear at first as small, round, highly refractive cells, but they swell rapidly in water with resulting rupture of the cell wall. In preparations stained with methylene blue the poly- morphic nucleus takes a strong stain. 2. Cornified cells {¥\g. 2,A^)- These IOO%i 50% o%J. I I I I 0 DAYS 10 19 Fig. 35.—Graph showing the percentages of each of the three types of cells in the vaginal smear of a mouse during the normal estrous cycle. Smears taken daily. cornified cells, nucleated epithelial cells, leukocytes. {Voss 1930.) are the largest cells in the smear. They are flattened, angular in outline, quite regular in size, and lack nuclei. 3. Nucleated epithelial cells (Fig. 34A). The typical epithelial cell is round, oval or polygonal, with clear cytoplasm and a centrally placed nucleus that takes a strong methylene blue stain. A number of variations occur. As estrus approaches the smear may contain epithelial cells with dark staining cytoplasm and karyolytic nuclei. The cytoplasm may contain droplets (of mucus?). A highly modified mucus- secreting type also occurs (no). This characteristically is goblet-shaped with the nucleus at the apex. The presence of mucus can be proved by the use of appropriate stains. Cells intermediate between cornified cells and nucleated epithelial cells occasionally occur. More or less mucus may occur in the smear. Different accounts dift"er greatly as to the amount normally present. It is possible that smearing or other forms of irritati
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