Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . etiologic factor of this disease is thecoccidioides immitis, an organism somewhat similar to but differing distinctly PARASITES. 171 in many respects from the blastomyces. This organism was first reported byPosadas and Wernicke, but was later classified by Rixford and Gilchrist andcultivated by Ophiils. 1 (7). Sporothrix organism is the cause of sporotrichosis and was first recognizedby Schenck, Hektoen and Perkins.^ Since its discovery a large number ofcases, have been
Diagnostic methods, chemical, bacteriological and microscopical, a text-book for students and practitioners . etiologic factor of this disease is thecoccidioides immitis, an organism somewhat similar to but differing distinctly PARASITES. 171 in many respects from the blastomyces. This organism was first reported byPosadas and Wernicke, but was later classified by Rixford and Gilchrist andcultivated by Ophiils. 1 (7). Sporothrix organism is the cause of sporotrichosis and was first recognizedby Schenck, Hektoen and Perkins.^ Since its discovery a large number ofcases, have been identified in all parts of the world, especially in the middle-western portions of the United States. The lesions in this conditionresemble, at times, those of tuberculosis, while, occasionally, they appearmore like those of syphilis. The chief characteristics are the sharplydefined, painless, cutaneous or subcutaneous abscesses which follow the lym-phatics and do not yield to the ordinary surgical procedures. In living tissuesthe sporothrix organisms appear as elongated or oval bodies fairly uniform in. Fig. 69.—Budding forms of Sporothrix Schenckii in pus, X 1200. Grams Stain. (Cour-tesy of Dr. D. J. Davis.) size and often showing distinct budding processes. The arrangement is usuallysingle, but frequently two or more are found end to end or arranged radiallyabout a central spore. Branched mycelial filaments are not found in the livingtissues but they develop abundantly on artificial media, forming, at their sidesand extremities, numerous spores, oval in shape and differing decidedly inappearance from the typical tissue forms. These latter forms may be found,however, in small numbers in artificial cultures (Davis). Smears from thelesions are made in the usual way. To obtain cultures proceed as follows:Some of the light yellow pus is taken, by aseptic methods, from the suspectedlesion, is transferred either to glucose or maltose agar, or blood agar, and is keptat room temper
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