. The diagnosis and treatment of diseases of women. at is too thick for the use of the oil-immersion lens. The cover-glasses may be STAINING THE GONOCCOCUS 389 kept in alcohol in a flat wide-mouthed bottle, from which they are removed anddried (cleaned) as needed. In the methylene-blue specimen, the nucleus of each pus cell is stained a lightblue. These nuclei are very irregular in shape and some of them are broken intotwo or more parts. They form the prominent light blue masses which largelyoccupy the field. The protoplasm, or body, or each cell is stained only very faintly,so faintly that it
. The diagnosis and treatment of diseases of women. at is too thick for the use of the oil-immersion lens. The cover-glasses may be STAINING THE GONOCCOCUS 389 kept in alcohol in a flat wide-mouthed bottle, from which they are removed anddried (cleaned) as needed. In the methylene-blue specimen, the nucleus of each pus cell is stained a lightblue. These nuclei are very irregular in shape and some of them are broken intotwo or more parts. They form the prominent light blue masses which largelyoccupy the field. The protoplasm, or body, or each cell is stained only very faintly,so faintly that it is ill-defined and hardly noticeable. All bacteria taking the stain,including the gonococci, are stained a very dark blue (almost black) and contrastwell with the hght blue nuclear masses. In vaginal specimens, the field is so filled with bacteria of various shapes andsizes,^that the gonococci are more or less obscured. In urethral specimens, how-ever, there are as a rule but few other bacteria and consequently the gonococci aremore easily Fig. 479. Specimen of pus from a case of Gonorrhoea,stained with methylene-blue. This field contains twogonococcus-colonies, each within a pus cell. Only thenuclei of the pus cells are seen. The lower colony has thecircular outline of the cell containing it. (Kolle and Was-sermann—Handhuch der Pathogenen Mikroorganismen), na €>0 Fig. 480. Indicating the Shape ofthe diplococcus of gonorrhoea (Gono-coccus). (Byford—Manual of Gynec-ology.) In acute gonorrhoea the gonococci are seen lying in colonies in the pus cells(Fig. 479) with a few scattered between the cells. They occur as diplococci, thetwo together having about the shape of two coffee grains with their flat surfacesturned toward each other and slightly separated (Fig. 480). They are spoken ofas biscuit-shaped or roll-shaped. The occurrence of the gonococci in small detached groups (Fig. 479), is a strikingfeature in a good specimen. The little colonies occur inside the
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