. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. I TOBACCO WILDFIRE IN 1922. 3 been made in regard to the life history of the causal organism, especially with reference to overwintering and dissemination. The results of the life history and control work of 1922 are briefly presented in the present bulletin. Valuable assistance in the work has been rendered by Prof. A. V. Osmun of the Massachusetts Experiment Station and Mr. C. M. Slagg of the United States Department of Agriculture. Tobacco growers in both States, too numerous to mention here by name, h
. Bulletin - Massachusetts Agricultural Experiment Station. Agriculture -- Massachusetts. I TOBACCO WILDFIRE IN 1922. 3 been made in regard to the life history of the causal organism, especially with reference to overwintering and dissemination. The results of the life history and control work of 1922 are briefly presented in the present bulletin. Valuable assistance in the work has been rendered by Prof. A. V. Osmun of the Massachusetts Experiment Station and Mr. C. M. Slagg of the United States Department of Agriculture. Tobacco growers in both States, too numerous to mention here by name, have co-operated heartily with the writers in the work described in the following pages. LIFE HISTORY STUDIES. Overwintering of the Bacteria. As a basis for control measures, probably no problem in regard to life history of the causal organism^ is more important than determination of the method or methods by which the bacteria survive the winter and thus serve as starting points for wildfire of the next year. Certain experiments with the object of solving this problem were conducted during the winter of 1921-22, and though some of the results are not conclusive progress to date is reported at this time. Other experiments with the same object are now in progress, and it is hoped that they will be more satisfactory. Effect of Freezing the Bacteria, ^^^ ^ _ ^ ^^^^p ^^ ^^^ ^^_ In stud^-ing the problem of overwintering, If^ ^^'f <=*"«.« wM&ie. ,„ . ,, .,., ^ Magmfied 5,C00 times. the first pomt to be deterrmned is the effect which freezing has on the organisms. If they are not able to withstand the exposure of a New England winter, then the measures of control will be quite different from those which should be tried if they are resistant to cold. Pure cultures of Bacterium tahacum on agar were placed out of doors at various times during the winter of 1921-22, some of them being frozen sohd for months; but in every case when they were brought back ' Wildfire is produce
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